Human IL17D

Mouse

Human (tested or 100% immunogen sequence identity)

IgG2b Monoclonal

246018

Unconjugated

Protein G purified

Unmodified

E. coli-derived recombinant human Interleukin 27 (aa 18-202).

aa18-202

Recognizes human IL-27. Does not cross-react with rhIL-17, rhIL17B, rhIL-17C, rhIL-17E, or rhIL-17F. Species sequence homology: Mouse: 86%.

• IHC
• IHC - Paraffin
• Western blot (1 - 2 µg/ml)
• Flow Cytometry (25 µg/ml)
• ELISA (0.5 - 1 µg/ml)

Suitable for use in Western Blot, Flow Cytometry, Immunohistochemistry and ELISA. Western Blot: 1-2 ug/ml. Detection limit is approximately 25 ng/lane under non-reducing conditions. Reducing conditions are not recommended. Flow Cytometry: 25 ug/ml. For intracellular staining, cells must be fixed and permeabilized using 4% paraformaldehyde and 0.1% saponin in PBS. Add 10 ul of the diluted solution to 1-5x10^5 cells in a total reaction volume not exceeding 200ul. Incubate for 30 minutes and then wash cells with 0.1% saponin prior to addition of a secondary developing reagent. Cells should be washed a final time in 0.1% saponin prior to flow cytometric analysis. Direct ELISA: 0.5-1 ug/ml. Detection limit is ~5 ng/well. Immunohistochemistry: 8-25 ug/ml with appropriate secondary reagents to detect IL-17D in paraffin-embedded normal heart tissue sections.

Lyophilized from PBS, 5% Trehalose

Reconstitute with sterile 40-50% glycerol, PBS.

Store at -20°C lyophilized. Once reconstituted, aliquot and store at -20°C.

For research use only. Intended for use by laboratory professionals.

This antibody carries the LSBio 100% Guarantee.

Uniprot: Q8TAD2 NCBI: NM_138284 NP_612141.1