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Catalog Number Size Price
LS-C104666-50 50 µg $356 
LS-C104666-100 100 µg $396 
IL1A / IL-1 Alpha Antibody - Western Blot (reducing) of IL1A / IL1 Alpha antibody. This image was taken for the unconjugated form of this product. Other forms have not been tested.
IL1A / IL-1 Alpha Antibody - Western Blot (non-reducing) of IL1A / IL1 Alpha antibody. This image was taken for the unconjugated form of this product. Other forms have not been tested.
IL1A / IL-1 Alpha Antibody - Sandwich ELISA of IL1A / IL1 Alpha antibody. This image was taken for the unconjugated form of this product. Other forms have not been tested.
IL1A / IL-1 Alpha Antibody - Western Blot (reducing) of IL1A / IL1 Alpha antibody. This image was taken for the unconjugated form of this product. Other forms have not been tested.
IL1A / IL-1 Alpha Antibody - Western Blot (non-reducing) of IL1A / IL1 Alpha antibody. This image was taken for the unconjugated form of this product. Other forms have not been tested.
IL1A / IL-1 Alpha Antibody - Sandwich ELISA of IL1A / IL1 Alpha antibody. This image was taken for the unconjugated form of this product. Other forms have not been tested.
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Polyclonal Rabbit anti‑Mouse IL1A / IL‑1 Alpha Antibody (WB) LS‑C104666

Polyclonal Rabbit anti‑Mouse IL1A / IL‑1 Alpha Antibody (WB) LS‑C104666

Note: This antibody replaces LS-C7681, LS-C54103, LS-C42229
Antibody:
IL1A / IL-1 Alpha Rabbit anti-Mouse Polyclonal Antibody
Application:
WB, ELISA
Reactivity:
Mouse
Format:
Unconjugated, Unmodified
Other formats:
Price
Catalog Number
$356
LS-C104666-50
Toll Free North America
206-374-1102
For Research Use Only

Overview

Antibody:
IL1A / IL-1 Alpha Rabbit anti-Mouse Polyclonal Antibody
Application:
WB, ELISA
Reactivity:
Mouse
Format:
Unconjugated, Unmodified
Other formats:

Specifications

Description
IL-1 Alpha antibody LS-C104666 is an unconjugated rabbit polyclonal antibody to mouse IL-1 Alpha (IL1A). Validated for ELISA and WB.
Target
Mouse IL1A / IL-1 Alpha
Synonyms
IL1A | Hematopoietin-1 | IL-1 alpha | IL1 | IL1-ALPHA | IL1 Alpha | Interleukin-1 alpha | IL-1A | IL1F1 | Pro-interleukin-1-alpha | Preinterleukin 1 alpha | Interleukin 1, alpha
Host
Rabbit
Reactivity
Mouse (tested or 100% immunogen sequence identity)
Clonality
Polyclonal
Conjugations
Unconjugated. Also available conjugated with Biotin.
Purification
Immunoaffinity purified
Modifications
Unmodified
Immunogen
E.coli derived recombinant Murine IL-1alpha.
Specificity
Mouse IL-1alpha
Applications
  • Western blot (0.1 - 0.2 µg/ml)
  • ELISA (0.5 - 2 µg/ml)
Usage
Immunohistochemistry: This antibody stained colchicine injected mouse brain (including the hippocampal fissure) tissue. The primary antibody was incubated at 1 mg/ml overnight at 4°C. This was followed by a peroxidase conjugated secondary antibody and then a fluorescein Tyramide Signal Amplification (TSA) reagent. Optimal concentrations and conditions may vary. Neutralization: To yield one-half maximal inhibition [ND] of the biological activity of mIL-1alpha (0.05 ng/ml), a concentration of 0 3-0.05 ug/ml of this antibody is required. ELISA: To detect mIL-1alpha by sandwich ELISA (using 100 ul/well antibody solution) a concentration of 0.5-2 ug/ml of this antibody is required. This antigen affinity purified antibody, in conjunction with Biotinylated Anti-Murine IL-1alpha (LS-C104815) as a detection antibody, allows the detection of at least 0.2-0.4 ng/well of recombinant mIL-1alpha. Western Blot: To detect mIL-1alpha by Western Blot analysis this antibody can be used at a concentration of 0.1-0.2 ug/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant mIL-1alpha is 1.5-3 ng/lane, under either reducing or non-reducing conditions.
Presentation
Lyophilized from PBS, pH 7.2
Reconstitution
Reconstitute with sterile water
Storage
Store Lyophilized at room temperature up to 1 month; Reconstituted for up to 2 weeks at 2°C to 8°C. Aliquot and freeze at -20°C for long term storage. Avoid freeze/thaw cycles.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee
About IL1A / IL-1 Alpha
P01583 NM_000575 NP_000566.3

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Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at:

Technical.Support@LSBio.com

Requested From: United States
Date Requested: 7/25/2024