Human IL3

Rat

Human (tested or 100% immunogen sequence identity)

IgG1,k Monoclonal

Unconjugated

Purified

Azide-free

Yeast-expressed recombinant human IL-3.

Recognizes human Interleukin-3 (IL-3).

• Western blot
• ELISA
• Neutralization

ELISA Capture: Purified monoclonal antibody is the preferred capture antibody in a sandwich ELISA for quantifying human IL-3 protein levels.1-3 The purified antibody should be paired with biotinylated antibody as the detection antibody, with purified recombinant human IL-3 as the standard. Western Blot: The purified antibody is also useful for Western blotting applications. A concentration of 1-5 ug/ml (in conjunction with AP-labeled Goat Anti-Rat-Ig) has been found to enable detection of 100 ng/lane of recombinant human IL-3 under reducing conditions. Neutralization: The LE/AF form of this antibody can be used for neutralizing human IL-3 bioactivity. A suitable LE/AF Rat IgG1 should be used as an isotype control. The applications listed have been tested for the unmodified form of this product. Other forms have not been tested.

PBS, No preservatives added

Short term: 4°C; Long term: Add glycerol (40-50%) -20°C.

For research use only. Intended for use by laboratory professionals.

This antibody carries the LSBio 100% Guarantee.

Uniprot: P08700 NCBI: NM_000588 NP_000579.2