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Antibodies > JUN / c-Jun > LS-C352425
 
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Catalog Number Size Price
LS-C352425-100 100 µl (1 mg/ml) $299 
JUN / c-Jun Antibody - Immunohistochemical analysis of c-Jun staining in human breast cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with hematoxylin and mounted with DPX.
JUN / c-Jun Antibody - Western blot analysis of c-Jun expression in HeLa (A); U2OS (B); HCT116 (C); NIH3T3 (D); PC12 (E); rat kidney (F) whole cell lysates.
JUN / c-Jun Antibody - Immunoprecipitation of c-Jun from 0.5mg HEK293T whole cell extract lysate using 5ug of Anti-c-Jun Antibody and 50ul of protein G magnetic beads (+). No antibody was added to the control (-). The antibody was incubated under agitation with Protein G beads for 10min HEK293T whole cell extract lysate diluted in RIPA buffer was added to each sample and incubated for a further 10min under agitation. Proteins were eluted by addition of 40ul SDS loading buffer and incubated for 10min at 70 C; 10ul of each sample was separated on a SDS PAGE gel transferred to a nitrocellulose membrane blocked with 5% BSA and probed with Anti-c-Jun Antibody.
JUN / c-Jun Antibody - ChIP analysis of Human endothelial cells (EA.hy926) incubated for 10 hours at 4 C. Cross-linking (X-ChIP) using formaldehyde for 10 minutes. Positive control: Position 89340150-89340297 in chromosome 11 (has a validated c-Jun site). Negative Control: Igr5 intron 3 (contains no c-Jun binding site). Detection step: Real-time PCR.
JUN / c-Jun Antibody - Immunohistochemical analysis of c-Jun staining in human breast cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with hematoxylin and mounted with DPX.
JUN / c-Jun Antibody - Western blot analysis of c-Jun expression in HeLa (A); U2OS (B); HCT116 (C); NIH3T3 (D); PC12 (E); rat kidney (F) whole cell lysates.
JUN / c-Jun Antibody - Immunoprecipitation of c-Jun from 0.5mg HEK293T whole cell extract lysate using 5ug of Anti-c-Jun Antibody and 50ul of protein G magnetic beads (+). No antibody was added to the control (-). The antibody was incubated under agitation with Protein G beads for 10min HEK293T whole cell extract lysate diluted in RIPA buffer was added to each sample and incubated for a further 10min under agitation. Proteins were eluted by addition of 40ul SDS loading buffer and incubated for 10min at 70 C; 10ul of each sample was separated on a SDS PAGE gel transferred to a nitrocellulose membrane blocked with 5% BSA and probed with Anti-c-Jun Antibody.
JUN / c-Jun Antibody - ChIP analysis of Human endothelial cells (EA.hy926) incubated for 10 hours at 4 C. Cross-linking (X-ChIP) using formaldehyde for 10 minutes. Positive control: Position 89340150-89340297 in chromosome 11 (has a validated c-Jun site). Negative Control: Igr5 intron 3 (contains no c-Jun binding site). Detection step: Real-time PCR.
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Polyclonal Rabbit anti‑Human JUN / c‑Jun Antibody (Internal, IHC, WB) LS‑C352425

Polyclonal Rabbit anti‑Human JUN / c‑Jun Antibody (Internal, IHC, WB) LS‑C352425

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Antibody:
JUN / c-Jun Rabbit anti-Human Polyclonal (Internal) Antibody
Application:
IHC, IHC-P, WB, IP, Flo, ChrIP
Reactivity:
Human, Mouse, Rat, Bovine, Pig, Chicken
Format:
Unconjugated, Unmodified
Price
Catalog Number
$299
LS-C352425-100
Toll Free North America
(800) 227-6666
For Research Use Only
Specifications Validation Publications Reviews Featured Products Request SDS/MSDS

Overview

Antibody:
JUN / c-Jun Rabbit anti-Human Polyclonal (Internal) Antibody
Application:
IHC, IHC-P, WB, IP, Flo, ChrIP
Reactivity:
Human, Mouse, Rat, Bovine, Pig, Chicken
Format:
Unconjugated, Unmodified

Specifications

Description
C-Jun antibody LS-C352425 is an unconjugated rabbit polyclonal antibody to c-Jun (JUN) (Internal) from human. It is reactive with human, mouse, rat and other species. Validated for ChrIP, Flow, IHC, IP and WB.
Target
Human JUN / c-Jun
Synonyms
JUN | Activator protein 1 | AP-1 | C-Jun | Jun proto-oncogene | p39 | Jun oncogene | Proto-oncogene c-Jun | AP1 | Enhancer-binding protein AP1 | G0s7 | Transcription factor AP-1 | v-jun
Host
Rabbit
Reactivity
Human, Mouse, Rat, Bovine, Pig, Chicken (tested or 100% immunogen sequence identity)
Clonality
Polyclonal
Conjugations
Unconjugated
Purification
Immunoaffinity purified
Modifications
Unmodified
Immunogen
KLH-conjugated synthetic peptide encompassing a sequence within the central region of human c-Jun.
Epitope
Internal
Specificity
Recognizes endogenous levels of c-Jun protein.
Applications
  • IHC
  • IHC - Paraffin (1:100 - 1:200)
  • Western blot (1:500 - 1:1000)
  • Immunoprecipitation (1:10 - 1:100)
  • Flow Cytometry (1:100 - 1:300)
  • Chromatin Immunoprecipitation
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Presentation
PBS, pH 7.3, 0.01% Sodium Azide, 30% Glycerol
Storage
Store at -20°C. Aliquot to avoid freeze/thaw cycles.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee
About JUN / c-Jun
P05412 NM_002228 NP_002219.1

Publications (0)

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JUN / c-Jun Antibody - Anti-JUN antibody IHC of human brain, cortex. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody dilution 1:100.
PathPlus™ JUN / c‑Jun Antibody (aa210‑259) LS‑B7572
Species: Human, Mouse, Rat
Applications: IHC, IHC - Paraffin, Western blot, Immunoprecipitation, Peptide Enzyme-Linked Immunosorbent Assay
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JUN / c‑Jun Antibody (phospho‑Ser73) LS‑C123254
Species: Human, Monkey, Mouse, Rat, Bovine, Dog, Pig, Rabbit, Sheep, Chicken, Xenopus, Zebrafish
Applications: IHC, Western blot
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JUN / c‑Jun Antibody LS‑C123260
Species: Human, Monkey, Mouse, Rat, Bovine, Dog, Pig, Rabbit, Sheep, Chicken, Xenopus, Zebrafish
Applications: IHC, Immunofluorescence, Western blot
JUN / c-Jun Antibody - Western blot of c-Jun phosphorylation expression in UV treated HeLa whole cell lysates,The lane on the left is treated with the antigen-specific peptide.
JUN / c‑Jun Antibody (phospho‑Thr239) LS‑C416473
Species: Human, Mouse, Rat
Applications: IHC, Western blot
JUN / c-Jun Antibody - Immunohistochemical analysis of c-Jun (pS63) staining in human breast cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with hematoxylin and mounted with DPX.
JUN / c‑Jun Antibody (phospho‑Ser63) LS‑C368401
Species: Human, Mouse, Rat, Bovine, Rabbit, Sheep
Applications: IHC, IHC - Paraffin, Western blot
JUN / c-Jun Antibody - Western blot of Acetyl-AP-1 (K271) antibody
JUN / c‑Jun Antibody (Internal) LS‑C387621
Species: Human, Mouse, Rat
Applications: Western blot, ELISA
See all JUN / c-Jun Antibodies

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at:

Technical.Support@LSBio.com
Requested From: United States
Date Requested: 12/27/2024
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Vector Laboratories, Inc.
6737 Mowry Ave
Newark, CA 94560
United States

Tel: (800) 227-6666 / (650) 697-3600

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For technical assistance:
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