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Catalog Number Size Price
LS-C413194-100 100 µl (1 mg/ml) $321 
JUN / c-Jun Antibody - Immunohistochemical analysis of c-Jun staining in human lung cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP polymer system. DAB was used as the chromogen. The section was then counterstained with hematoxylin and mounted with DPX.
JUN / c-Jun Antibody - Immunofluorescent analysis of c-Jun staining in HeLa cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 deg C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue).
JUN / c-Jun Antibody - Western blot analysis of c-Jun expression in HeLa (A); HUVEC (B) whole cell lysates.
JUN / c-Jun Antibody - Immunohistochemical analysis of c-Jun staining in human lung cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP polymer system. DAB was used as the chromogen. The section was then counterstained with hematoxylin and mounted with DPX.
JUN / c-Jun Antibody - Immunofluorescent analysis of c-Jun staining in HeLa cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 deg C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue).
JUN / c-Jun Antibody - Western blot analysis of c-Jun expression in HeLa (A); HUVEC (B) whole cell lysates.
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Polyclonal Rabbit anti‑Human JUN / c‑Jun Antibody (Internal, IHC, IF, WB) LS‑C413194

Polyclonal Rabbit anti‑Human JUN / c‑Jun Antibody (Internal, IHC, IF, WB) LS‑C413194

Antibody:
JUN / c-Jun Rabbit anti-Human Polyclonal (Internal) Antibody
Application:
IHC, IHC-P, ICC, IF, WB
Reactivity:
Human, Mouse, Rat, Bovine, Pig, Chicken
Format:
Unconjugated, Unmodified
Price
Catalog Number
$321
LS-C413194-100
Toll Free North America
(800) 227-6666
For Research Use Only

Overview

Antibody:
JUN / c-Jun Rabbit anti-Human Polyclonal (Internal) Antibody
Application:
IHC, IHC-P, ICC, IF, WB
Reactivity:
Human, Mouse, Rat, Bovine, Pig, Chicken
Format:
Unconjugated, Unmodified

Specifications

Description
C-Jun antibody LS-C413194 is an unconjugated rabbit polyclonal antibody to c-Jun (JUN) (Internal) from human. It is reactive with human, mouse, rat and other species. Validated for ICC, IF, IHC and WB.
Target
Human JUN / c-Jun
Synonyms
JUN | Activator protein 1 | AP-1 | C-Jun | Jun proto-oncogene | p39 | Jun oncogene | Proto-oncogene c-Jun | AP1 | Enhancer-binding protein AP1 | G0s7 | Transcription factor AP-1 | v-jun
Host
Rabbit
Reactivity
Human, Mouse, Rat, Bovine, Pig, Chicken (tested or 100% immunogen sequence identity)
Clonality
Polyclonal
Conjugations
Unconjugated
Purification
Immunoaffinity purified
Modifications
Unmodified
Immunogen
KLH-conjugated synthetic peptide encompassing a sequence within the central region of human c-Jun.
Epitope
Internal
Specificity
Recognizes endogenous levels of c-Jun protein.
Applications
  • IHC
  • IHC - Paraffin (1:100 - 1:200)
  • ICC (1:100 - 1:500)
  • Immunofluorescence (1:100 - 1:500)
  • Western blot (1:500 - 1:1000)
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Presentation
PBS, pH 7.3, 0.01% Sodium Azide, 30% Glycerol
Storage
Aliquot and store at -20°C for 1 year. Avoid freeze/thaw cycles.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee
About JUN / c-Jun
P05412 NM_002228 NP_002219.1

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To request an SDS/MSDS form for this product, please contact our Technical Support department at:

Technical.Support@LSBio.com

Requested From: United States
Date Requested: 11/22/2024