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Catalog Number	Size	Price
LS-C368396-30	30 µl (1 mg/ml)	$263
LS-C368396-100	100 µl (1 mg/ml)	$315
LS-C368396-200	200 µl (1 mg/ml)	$379

JUN / c-Jun Antibody - Immunohistochemical analysis of c-Jun (pT239) staining in human breast cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with hematoxylin and mounted with DPX.

JUN / c-Jun Antibody - Immunofluorescent analysis of c-Jun (pT239) staining in HeLa cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 deg C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue).

JUN / c-Jun Antibody - Western blot analysis of c-Jun (pT239) expression in HEK293T UV-treated (A); HeLa UV-treated (B) whole cell lysates.

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Polyclonal Rabbit anti‑Human JUN / c‑Jun Antibody (phospho‑Thr239, IHC, IF, WB) LS‑C368396

Name: Anti-JUN / c-Jun Antibody | Rabbit anti-Human Polyclonal | LSBio
Brand: Lifespan BioSciences
SKU: LS-C368396-30
Price: 263.0000 USD
Availability: InStock

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Antibody:

JUN / c-Jun Rabbit anti-Human Polyclonal (pThr239) Antibody

Application:

IHC, IHC-P, ICC, IF, WB, IP

Reactivity:

Human, Mouse, Rat, Bovine, Pig, Chicken

Format:

Unconjugated, Unmodified

$263

LS-C368396-30

$315

LS-C368396-100

$379

LS-C368396-200

Toll Free North America

(800) 227-6666

For Research Use Only

Specifications Validation Publications Reviews Featured Products Request SDS/MSDS

Overview

Antibody:

JUN / c-Jun Rabbit anti-Human Polyclonal (pThr239) Antibody

Application:

IHC, IHC-P, ICC, IF, WB, IP

Reactivity:

Human, Mouse, Rat, Bovine, Pig, Chicken

Format:

Unconjugated, Unmodified

Specifications

Description

C-Jun antibody LS-C368396 is an unconjugated rabbit polyclonal antibody to c-Jun (JUN) (pThr239) from human. It is reactive with human, mouse, rat and other species. Validated for ICC, IF, IHC, IP and WB.

Target

Human JUN / c-Jun

Synonyms

See All JUN / c-Jun Antibodies

Host

Rabbit

Reactivity

Human, Mouse, Rat, Bovine, Pig, Chicken (tested or 100% immunogen sequence identity)

Clonality

Polyclonal

Conjugations

Unconjugated

Purification

Immunoaffinity purified

Modifications

Unmodified

Immunogen

KLH-conjugated synthetic peptide encompassing a sequence within the central region of human c-Jun.

Epitope

pThr239

Specificity

Recognizes endogenous levels of c-Jun (pT239) protein.

Applications

IHC
IHC - Paraffin (1:100 - 1:200)
ICC (1:100 - 1:500)
Immunofluorescence
Western blot (1:500 - 1:1000)
Immunoprecipitation (1:10 - 1:100)

Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.

Presentation

PBS, pH 7.3, 0.01% Sodium Azide, 30% Glycerol

Storage

Aliquot and store at -20°C for 1 year. Avoid freeze/thaw cycles.

Restrictions

For research use only. Intended for use by laboratory professionals.

Guarantee

This antibody carries the LSBio 100% Guarantee.

LSBio Guarantee

About JUN / c-Jun

Uniprot: P05412 NCBI: NM_002228 NP_002219.1