Human KITLG / SCF

Mouse

Human (tested or 100% immunogen sequence identity)

IgG1 Monoclonal

Unconjugated

Protein G purified

Unmodified

E.coli derived recombinant Human SCF.

Human SCF

• Western blot (0.25 - 0.5 µg/ml)
• ELISA (4 - 8 µg/ml)

Immunohistochemistry: This antibody stained CACO-2 cells and A-431cells. The primary antibody was incubated at 2 ug/ml overnight at 4°C followed by a fluorescent labeled secondary antibody. Optimal concentrations and conditions may vary. Information and photo are courtesy of the Cell Profiling group, SciLifeLab Stockholm. ELISA: In a sandwich ELISA (assuming 100 ul/well), a concentration of 4-8 ug/ml of this antibody will detect at least 1000 pg/ml of recombinant human SCF when used with biotinylated antigen affinity purified anti-human SCF (LS-C104672) as the detection antibody at a concentration of approximately 1-2 ug/ml. Western Blot: To detect hSCF by Western Blot analysis this antibody can be used at a concentration of 0.25-0.50 ug/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant hSCF is 2-4 ng/lane, under non-reducing conditions.

Lyophilized from PBS

Reconstitute with sterile water to a concentration of 1 mg/ml.

Store Lyophilized at room temperature up to 1 month; Reconstituted for up to 2 weeks at 2°C to 8°C. Aliquot and freeze at -20°C for long term storage. Avoid freeze/thaw cycles.

For research use only. Intended for use by laboratory professionals.

This antibody carries the LSBio 100% Guarantee.

Uniprot: P21583 NCBI: NM_000899 NP_000890.1