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Catalog Number	Size	Price
LS-C800277-100	100 µl (1 mg/ml)	$379
LS-C800277-200	200 µl (1 mg/ml)	$421

MAPK3 / ERK1 Antibody - 1:50 staining human colon carcinoma tissue by IHC-P. The tissue was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The tissue was then blocked and incubated with the antibody for 1.5 hours at 22°C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.

MAPK3 / ERK1 Antibody - Staining LOVO cells by IF/ICC. The samples were fixed with PFA and permeabilized in 0.1% saponin prior to blocking in 10% serum for 45 min at 37°C. The primary antibody was diluted 1/400 and incubated with the sample for 1 hour at 37°C. A Alexa Fluor® 594 conjugated goat polyclonal to rabbit IgG (H+L), diluted 1/600 was used as secondary antibody.

MAPK3 / ERK1 Antibody - Western blot analysis on COLO205 cell lysates using ERK1/2 antibody. The lane on the left is treated with the antigen-specific peptide.

MAPK3 / ERK1 Antibody - Western blot analysis of extracts of various cellslines using erk1/2 antibody.

MAPK3 / ERK1 Antibody - Western blot analysis on HeLa cell lysates using ERK1/2 antibody

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Polyclonal Rabbit anti‑Human MAPK3 / ERK1 Antibody (IHC, IF, WB) LS‑C800277

Name: Anti-MAPK3 / ERK1 Antibody | Rabbit anti-Human Polyclonal | LSBio
Brand: Lifespan BioSciences
SKU: LS-C800277-100
Price: 379.0000 USD
Availability: InStock

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Antibody:

MAPK3 / ERK1 Rabbit anti-Human Polyclonal Antibody

Application:

IHC, IF, WB, Peptide-ELISA

Reactivity:

Human, Monkey, Mouse, Rat, Bovine, Dog, Horse, Pig, Sheep, Fish, Zebrafish

Format:

Unconjugated, Unmodified

$379

LS-C800277-100

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Overview

Antibody:

MAPK3 / ERK1 Rabbit anti-Human Polyclonal Antibody

Application:

IHC, IF, WB, Peptide-ELISA

Reactivity:

Human, Monkey, Mouse, Rat, Bovine, Dog, Horse, Pig, Sheep, Fish, Zebrafish

Format:

Unconjugated, Unmodified

Specifications

Description

ERK1 antibody LS-C800277 is an unconjugated rabbit polyclonal antibody to ERK1 (MAPK3) from human. It is reactive with human, mouse, rat and other species. Validated for IF, IHC, Peptide-ELISA and WB.

Target

Human MAPK3 / ERK1

Synonyms

See All MAPK3 / ERK1 Antibodies

Host

Rabbit

Reactivity

Human, Monkey, Mouse, Rat, Bovine, Dog, Horse, Pig, Sheep, Fish, Zebrafish (tested or 100% immunogen sequence identity)

Clonality

IgG Polyclonal

Conjugations

Unconjugated

Purification

Peptide affinity chromatography.

Modifications

Unmodified

Immunogen

A synthesized peptide derived from human ERK1/2.

Specificity

ERK1/2 antibody detects endogenous levels of total ERK1/2.

Applications

IHC (1:100 - 1:500)
Immunofluorescence
Western blot (1:1000 - 1:5000)
Peptide Enzyme-Linked Immunosorbent Assay (1:20000 - 1:40000)

Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.

Usage

For western blots: incubate membrane with diluted antibody overnight in 5% w/v milk , 1X TBS, 0.1% Tween-20 at 4°C with gentle shaking.

Presentation

PBS, pH 7.4, 50% Glycerol

Storage

Upon receipt, store at -20°C. Avoid freeze-thaw cycles.

Restrictions

For research use only. Intended for use by laboratory professionals.

Guarantee

This antibody carries the LSBio 100% Guarantee.

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About MAPK3 / ERK1