Human MAPK3 / ERK1

Mouse

Human, Mouse, Rat, Hamster (tested or 100% immunogen sequence identity)

IgG1 Monoclonal

Unconjugated

Ascites

Unmodified

Synthetic phosphopeptide (KLH coupled) corresponding to residues around Thr202/Tyr204 of human p44 MAP kinase.

pThr202/Tyr204

Detects endogenous levels of human p44 and p42 MAP kinase (Erk1 and Erk2) dually phosphorylated at threonine 202 and tyrosine 204. Does not cross-react with the corresponding phosphorylated residues of either SAPK/JNK or p38 MAP kinase. Species cross-reactivity: Hamster, mouse, rat and zebrafish.

• ICC (1:200)
• Immunofluorescence (1:200)
• Western blot (1:2000)
• Immunoprecipitation (1:500)
• Flow Cytometry (1:1000)
• ELISA

Suitable for use in Flow Cytometry, ELISA, Western Blot, Immunoprecipitation, Immunofluorescence and Immunocytochemistry. Western Blot: 1:2000, incubate membrane with diluted antibody in 5% nonfat dry milk, 1X TBS, 0.1% Tween-20 at 4?C with gentle shaking, overnight. Immunoprecipitation: 1:500. Immunofluorescence: 1:200. Immunocytochemistry: 1:200. Flow Cytometry: 1:1000.

10 mM HEPES, pH 7.5, 150 mM NaCl, 0.1 mg/ml BSA, 0.02% Sodium Azide, 50% Glycerol

Short term: store at 4°C. Long term: store at -20°C. Avoid freeze-thaw cycles.

For research use only. Intended for use by laboratory professionals.

This antibody carries the LSBio 100% Guarantee.

Uniprot: P27361 NCBI: X60188 CAA42744.1