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Catalog Number	Size	Price
LS-C800702-100	100 µl (1 mg/ml)	$379
LS-C800702-200	200 µl (1 mg/ml)	$421

MAPK3 / ERK1 Antibody - 1:200 staining mouse liver tissue by IHC-P. The tissue was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The tissue was then blocked and incubated with the antibody for 1.5 hours at 22°C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.

MAPK3 / ERK1 Antibody - 1:200 staining rat lung tissue by IHC-P. The tissue was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The tissue was then blocked and incubated with the antibody for 1.5 hours at 22°C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.

MAPK3 / ERK1 Antibody - 1:200 staining human pancreas tissue by IHC-P. The tissue was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The tissue was then blocked and incubated with the antibody for 1.5 hours at 22°C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.

MAPK3 / ERK1 Antibody - Phospho-ERK1/2 (Thr202/Tyr204) antibody for IHC in human testis

MAPK3 / ERK1 Antibody - 1:200 staining human heart tissue by IHC-P. The tissue was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The tissue was then blocked and incubated with the antibody for 1.5 hours at 22°C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.

MAPK3 / ERK1 Antibody - Staining LOVO cells by IF/ICC. The samples were fixed with PFA and permeabilized in 0.1% saponin prior to blocking in 10% serum for 45 min at 37°C. The primary antibody was diluted 1/400 and incubated with the sample for 1 hour at 37°C. A Alexa Fluor® 594 conjugated goat polyclonal to rabbit IgG (H+L), diluted 1/600 was used as secondary antibody.

MAPK3 / ERK1 Antibody - Western blot analysis of ERK1/2 phosphorylation expression in HeLa whole cells lysates. The lane on the left is treated with the antigen-specific peptide.

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Polyclonal Rabbit anti‑Human MAPK3 / ERK1 Antibody (phospho‑Tyr204,phospho‑Thr202, IHC, IF, WB) LS‑C800702

Name: Anti-MAPK3 / ERK1 Antibody | Rabbit anti-Human Polyclonal | LSBio
Brand: Lifespan BioSciences
SKU: LS-C800702-100
Price: 379.0000 USD
Availability: InStock

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Antibody:

MAPK3 / ERK1 Rabbit anti-Human Polyclonal (pTyr204,pThr202) Antibody

Application:

IHC, IF, WB, Peptide-ELISA

Reactivity:

Human, Mouse, Rat

Format:

Unconjugated, Unmodified

$379

LS-C800702-100

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(800) 227-6666

For Research Use Only

Specifications Validation Publications Reviews Featured Products Request SDS/MSDS

Overview

Antibody:

MAPK3 / ERK1 Rabbit anti-Human Polyclonal (pTyr204,pThr202) Antibody

Application:

IHC, IF, WB, Peptide-ELISA

Reactivity:

Human, Mouse, Rat

Format:

Unconjugated, Unmodified

Specifications

Description

ERK1 antibody LS-C800702 is an unconjugated rabbit polyclonal antibody to ERK1 (MAPK3) (pTyr204,pThr202) from human. It is reactive with human, mouse and rat. Validated for IF, IHC, Peptide-ELISA and WB.

Target

Human MAPK3 / ERK1

Synonyms

See All MAPK3 / ERK1 Antibodies

Host

Rabbit

Reactivity

Human, Mouse, Rat (tested or 100% immunogen sequence identity)

Clonality

IgG Polyclonal

Conjugations

Unconjugated

Purification

Affinity purification via sequential chromatography on phospho- and non-phospho-peptide affinity columns.

Modifications

Unmodified

Immunogen

A synthesized peptide derived from human ERK1/2 around the phosphorylation site of Threonine 202/Tyrosine 204.

Epitope

pTyr204,pThr202

Specificity

Phospho-ERK1/2 (Thr202/Tyr204) Antibody detects endogenous levels of ERK1/2 only when phosphorylated at Threonine 202/Tyrosine 204.

Applications

IHC (1:50 - 1:200)
Immunofluorescence
Western blot (1:500 - 1:2000)
Peptide Enzyme-Linked Immunosorbent Assay (1:20000 - 1:40000)

Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.

Usage

For western blots: incubate membrane with diluted antibody overnight in 5% w/v milk , 1X TBS, 0.1% Tween-20 at 4°C with gentle shaking.

Presentation

PBS, pH 7.4, 0.02% Sodium Azide, 50% Glycerol

Storage

Upon receipt, store at -20°C. Avoid freeze-thaw cycles.

Restrictions

For research use only. Intended for use by laboratory professionals.

Guarantee

This antibody carries the LSBio 100% Guarantee.

LSBio Guarantee

About MAPK3 / ERK1