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Antibodies > MCAM / CD146 > LS-C675304
 
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Catalog Number Size Price
LS-C675304-50 50 µl $316 
LS-C675304-100 100 µl (0.25 mg/ml) $386 
MCAM / CD146 Antibody - Immunohistochemistry Dilution at 1:100 and staining in paraffin-embedded human breast cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal Goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated Secondary antibody and visualized using an HRP conjugated SP system.
MCAM / CD146 Antibody - Immunofluorescence staining of U251 cells diluted at 1:37,counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C.The Secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG (H+L).
MCAM / CD146 Antibody - Western Blot Positive WB detected in:A375 whole cell lysate All Lanes:CD146 antibody at 0.6µg/ml Secondary Goat polyclonal to rabbit IgG at 1/50000 dilution Predicted band size: 72 KDa Observed band size: 120 KDa
MCAM / CD146 Antibody - Overlay histogram showing A375 cells stained with CD146 Antibody (red line) at 1:50. The cells were fixed with 70% Ethylalcohol (18h) and then permeabilized with 0.3% Triton X-100 for 2 min.The cells were then incubated in 1x PBS /10% normal goat serum to block non-specific protein-protein interactions followed by primary antibody for 1 h at 4?.The secondary antibody used was FITC goat anti-rabbit IgG (H+L) at 1/200 dilution for 1 h at 4?. Control antibody (green line) was used under the same conditions. Acquisition of >10,000 events was performed.
MCAM / CD146 Antibody - Immunohistochemistry Dilution at 1:100 and staining in paraffin-embedded human breast cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal Goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated Secondary antibody and visualized using an HRP conjugated SP system.
MCAM / CD146 Antibody - Immunofluorescence staining of U251 cells diluted at 1:37,counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C.The Secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG (H+L).
MCAM / CD146 Antibody - Western Blot Positive WB detected in:A375 whole cell lysate All Lanes:CD146 antibody at 0.6µg/ml Secondary Goat polyclonal to rabbit IgG at 1/50000 dilution Predicted band size: 72 KDa Observed band size: 120 KDa
MCAM / CD146 Antibody - Overlay histogram showing A375 cells stained with CD146 Antibody (red line) at 1:50. The cells were fixed with 70% Ethylalcohol (18h) and then permeabilized with 0.3% Triton X-100 for 2 min.The cells were then incubated in 1x PBS /10% normal goat serum to block non-specific protein-protein interactions followed by primary antibody for 1 h at 4?.The secondary antibody used was FITC goat anti-rabbit IgG (H+L) at 1/200 dilution for 1 h at 4?. Control antibody (green line) was used under the same conditions. Acquisition of >10,000 events was performed.
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Monoclonal Rabbit anti‑Human MCAM / CD146 Antibody (IHC, IF, WB) LS‑C675304

Monoclonal Rabbit anti‑Human MCAM / CD146 Antibody (IHC, IF, WB) LS‑C675304

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Antibody:
MCAM / CD146 Rabbit anti-Human Monoclonal Antibody
Application:
IHC, IHC-P, IF, WB, Flo, ELISA
Reactivity:
Human
Format:
Unconjugated, Unmodified
Price
Catalog Number
$316
LS-C675304-50
Toll Free North America
(800) 227-6666
For Research Use Only
Specifications Validation Publications Reviews Featured Products Request SDS/MSDS

Overview

Antibody:
MCAM / CD146 Rabbit anti-Human Monoclonal Antibody
Application:
IHC, IHC-P, IF, WB, Flo, ELISA
Reactivity:
Human
Format:
Unconjugated, Unmodified

Specifications

Description
CD146 antibody LS-C675304 is an unconjugated rabbit monoclonal antibody to human CD146 (MCAM). Validated for ELISA, Flow, IF, IHC and WB.
Target
Human MCAM / CD146
Synonyms
MCAM | CD146 | CD146 antigen | Gicerin | Melanoma adhesion molecule | MUC18 | p1h12
Host
Rabbit
Reactivity
Human (tested or 100% immunogen sequence identity)
Clonality
IgG Monoclonal
Conjugations
Unconjugated
Purification
Affinity chromatography
Modifications
Unmodified
Immunogen
A synthesized peptide
Applications
  • IHC
  • IHC - Paraffin
  • Immunofluorescence
  • Western blot
  • Flow Cytometry
  • ELISA
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Presentation
PBS, pH 7.4, 150 mM NaCl, 0.02% Sodium Azide, 50% Glycerol
Storage
Short term: store at -20°C. Long term: store at -80°C. Avoid freeze-thaw cycles.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee
About MCAM / CD146
P43121 NM_006500 NP_006491.2

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IHC‑plus™ MCAM / CD146 Antibody (clone P1H12) LS‑B4050
Species: Human
Applications: IHC, IHC - Paraffin, IHC - Frozen, Western blot, Immunoprecipitation, Flow Cytometry
MCAM / CD146 Antibody - Anti-MCAM / CD146 antibody IHC staining of human placenta. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 10 ug/ml.
IHC‑plus™ MCAM / CD146 Antibody (Tyr641) LS‑B3628
Species: Human
Applications: IHC, IHC - Paraffin, Western blot
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Species: Human
Applications: IHC, Western blot, ELISA
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MCAM / CD146 Antibody (Extracellular Domain) LS‑C130676
Species: Mouse
Applications: Western blot, ELISA
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MCAM / CD146 Antibody (Biotin) LS‑C14263
Species: Human, Mouse, Dog, Rabbit
Applications: IHC, ICC, Immunoprecipitation, Flow Cytometry, ELISA
See all MCAM / CD146 Antibodies

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at:

Technical.Support@LSBio.com
Requested From: United States
Date Requested: 11/3/2024
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