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Vector Laboratories, Inc.
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Customer Service: (800) 227-6666 / (650) 697-3600


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Catalog Number Size Price
LS-C312808-10 10 µg $318 
LS-C312808-100 100 µg (0.5 mg/ml) $470 
MCM5 Antibody - MCM5 antibody. IHC(P): Human Intestinal Cancer Tissue.
MCM5 Antibody - Western blot analysis of MCM5 using anti-MCM5 antibody. Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human Jurkat whole cell lysates, After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-MCM5 antigen affinity purified polyclonal antibody at 0.5 µg/mL overnight at 4°C, then washed with TBS-0.1% Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for MCM5 at approximately 82KD. The expected band size for MCM5 is at 82KD.
MCM5 Antibody - WB of MCM5 antibody. All lanes: Anti-MCM5 at 0.5ug/ml. Lane 1: Rat Testis Tissue Lysate at 40ug. Lane 2: Rat Brain Tissue Lysate at 40ug. Lane 3: JURKAT Whole Cell Lysate at 40ug. Predicted bind size: 82KD. Observed bind size: 82KD.
MCM5 Antibody - MCM5 antibody. IHC(P): Human Intestinal Cancer Tissue.
MCM5 Antibody - Western blot analysis of MCM5 using anti-MCM5 antibody. Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human Jurkat whole cell lysates, After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-MCM5 antigen affinity purified polyclonal antibody at 0.5 µg/mL overnight at 4°C, then washed with TBS-0.1% Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for MCM5 at approximately 82KD. The expected band size for MCM5 is at 82KD.
MCM5 Antibody - WB of MCM5 antibody. All lanes: Anti-MCM5 at 0.5ug/ml. Lane 1: Rat Testis Tissue Lysate at 40ug. Lane 2: Rat Brain Tissue Lysate at 40ug. Lane 3: JURKAT Whole Cell Lysate at 40ug. Predicted bind size: 82KD. Observed bind size: 82KD.
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Polyclonal Rabbit anti‑Human MCM5 Antibody (aa720‑734, IHC, WB) LS‑C312808

Polyclonal Rabbit anti‑Human MCM5 Antibody (aa720‑734, IHC, WB) LS‑C312808

Note: This antibody replaces LS-C388812
Antibody:
MCM5 Rabbit anti-Human Polyclonal (aa720-734) Antibody
Application:
IHC, IHC-P, WB
Reactivity:
Human, Monkey, Mouse, Rat, Bat, Bovine
Format:
Unconjugated, Unmodified
Price
Catalog Number
$318
LS-C312808-10
Toll Free North America
(800) 227-6666
For Research Use Only

Overview

Antibody:
MCM5 Rabbit anti-Human Polyclonal (aa720-734) Antibody
Application:
IHC, IHC-P, WB
Reactivity:
Human, Monkey, Mouse, Rat, Bat, Bovine
Format:
Unconjugated, Unmodified

Specifications

Description
MCM5 antibody LS-C312808 is an unconjugated rabbit polyclonal antibody to MCM5 (aa720-734) from human. It is reactive with human, mouse, rat and other species. Validated for IHC and WB.
Target
Human MCM5
Synonyms
MCM5 | CDC46 | CDC46 homolog | p1-CDC46
Host
Rabbit
Reactivity
Human, Monkey, Mouse, Rat, Bat, Bovine (tested or 100% immunogen sequence identity)
Clonality
Polyclonal
Conjugations
Unconjugated
Purification
Immunogen affinity purified
Modifications
Unmodified
Immunogen
A synthetic peptide corresponding to a sequence at the C-Terminus of human MCM5(720-734 aa EIQHRMQRKVLYRLK), identical to the related rat and mouse sequences.
Epitope
aa720-734
Applications
  • IHC
  • IHC - Paraffin
  • Western blot
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Presentation
Lyophilized from 0.2mg Na2HPO4, 5mg BSA, 0.9mg NaCl, 0.05mg Thimerosal, 0.05mg sodium azide.
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500µg/ml.
Storage
At -20°C for 1 year. After reconstitution, at 4°C for 1 month. It can also be aliquotted and stored frozen at -20°C for a longer time. Avoid freeze-thaw cycles.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee
About MCM5
P33992 NM_006739 NP_006730.2

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Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at:

Technical.Support@LSBio.com

Requested From: United States
Date Requested: 11/22/2024