Target
Human MKI67 / Ki67
Synonyms
MKI67 | Antigen KI-67 | Ki67 | KIA
Reactivity
Human
(tested or 100% immunogen sequence identity)
Conjugations
Unconjugated
Purification
Protein A purified
Immunogen
Synthetic peptide corresponding to an internal region of human Ki-67 (within residues 1550-1700). Cellular Localization: Nuclear
Specificity
Recognizes human Ki-67. Reacts with a nuclear antigen present in proliferating human cells. Can be used to evaluate the Ki-67 labeling index in various tumors.
Applications
- IHC
- IHC - Paraffin
- IHC - Frozen
- ICC (1:50)
- Immunofluorescence (1:20)
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Usage
Suitable for use in Immunohistochemistry, Immunocytochemistry and Immunofluorescence. Immunohistochemistry (Frozen/Paraffin): Formalin-fixed paraffin-embedded tissue sections require high temperature antigen unmasking with 10 mM citrate buffer, pH 6.0 prior to immunostaining. This antibody will not work without optimal antigen retrieval. We suggest an incubation period of 30 minutes at RT and to use DAB to stain the protein (immunofluorescence may give problems as the protein is nuclear). Immunocytochemistry: 1:50. Immunofluorescence: 1:20. Positive control: Tonsil.
Presentation
PBS, 0.05% Sodium Azide, 30% Glycerol
Storage
Short term: store at 4°C. Long term: store at -20°C. Avoid freeze-thaw cycles.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee