Rat MMP9 / Gelatinase B

Rabbit

Rat, Human, Mouse (tested or 100% immunogen sequence identity)

IgG Polyclonal

Unconjugated. Also available conjugated with APC, Biotin, FITC, HRP, PE.

Protein A purified

Unmodified

E. coli expressed active rat 92 kDa type IV collagenase (catalytic domain)

Recognizes rat MMP-9 pro and active forms. Exhibits no cross-reactivity with other MMP family members.

• IHC
• IHC - Paraffin (5 µg/ml)
• IHC - Frozen (1:50)
• Western blot (1:1000)
• Immunoprecipitation (1:250)

Immunohistochemistry: LS-B2486 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B2486 was determined to be 5 ug/ml. Western Blot: 1:500. Under fully reduced conditions, typically 98-95kD pro and 92-88kD active forms and breakdown products are detected. Under non-reduced conditions, additional bands may be detected due to SDS-stable TIMP and other inhibitor complexes. Positive control: HL660 cell lysate, U937 cell lysate, lung.

PBS, 0.05% Sodium Azide

Short term: 4°C Long term: Aliquot and store at -20°C. Avoid freeze-thaw cycles.

For research use only. Intended for use by laboratory professionals.

This antibody carries the LSBio 100% Guarantee.

Uniprot: P14780 NCBI: NM_004994 NP_004985.2