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Catalog Number Size Price
LS-C352579-100 100 µl (1 mg/ml) $315 
MYC / c-Myc Antibody - Immunohistochemical analysis of c-Myc (pS62) staining in human breast cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with hematoxylin and mounted with DPX.
MYC / c-Myc Antibody - Immunofluorescent analysis of c-Myc (pS62) staining in HeLa cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue).
MYC / c-Myc Antibody - Western blot analysis of c-Myc (pS62) expression in A431 (A); HeLa (B); Jurkat (C) whole cell lysates.
MYC / c-Myc Antibody - Immunohistochemical analysis of c-Myc (pS62) staining in human breast cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with hematoxylin and mounted with DPX.
MYC / c-Myc Antibody - Immunofluorescent analysis of c-Myc (pS62) staining in HeLa cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue).
MYC / c-Myc Antibody - Western blot analysis of c-Myc (pS62) expression in A431 (A); HeLa (B); Jurkat (C) whole cell lysates.
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Polyclonal Rabbit anti‑Human MYC / c‑Myc Antibody (phospho‑Ser62, IHC, IF, WB) LS‑C352579

Polyclonal Rabbit anti‑Human MYC / c‑Myc Antibody (phospho‑Ser62, IHC, IF, WB) LS‑C352579

Antibody:
MYC / c-Myc Rabbit anti-Human Polyclonal (pSer62) Antibody
Application:
IHC, IHC-P, ICC, IF, WB
Reactivity:
Human, Mouse
Format:
Unconjugated, Unmodified
Price
Catalog Number
$315
LS-C352579-100
Toll Free North America
(800) 227-6666
For Research Use Only

Overview

Antibody:
MYC / c-Myc Rabbit anti-Human Polyclonal (pSer62) Antibody
Application:
IHC, IHC-P, ICC, IF, WB
Reactivity:
Human, Mouse
Format:
Unconjugated, Unmodified

Specifications

Description
C-Myc antibody LS-C352579 is an unconjugated rabbit polyclonal antibody to c-Myc (MYC) (pSer62) from human. It is reactive with human and mouse. Validated for ICC, IF, IHC and WB.
Target
Human MYC / c-Myc
Synonyms
MYC | BHLHe39 | C-Myc | MRTL | Myc proto-oncogene protein | Proto-oncogene c-Myc | Rats1 | Transcription factor p64 | v-myc
Host
Rabbit
Reactivity
Human, Mouse (tested or 100% immunogen sequence identity)
Clonality
Polyclonal
Conjugations
Unconjugated
Purification
Immunoaffinity purified
Modifications
Unmodified
Immunogen
KLH-conjugated synthetic peptide encompassing a sequence within the N-Terminus of human c-Myc.
Epitope
pSer62
Specificity
Recognizes endogenous levels of c-Myc (pS62) protein.
Applications
  • IHC
  • IHC - Paraffin (1:100 - 1:200)
  • ICC (1:100 - 1:500)
  • Immunofluorescence
  • Western blot (1:500 - 1:1000)
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Presentation
PBS, pH 7.3, 0.01% Sodium Azide, 30% Glycerol
Storage
Store at -20°C. Aliquot to avoid freeze/thaw cycles.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee
About MYC / c-Myc
P01106 NM_002467 NP_002458.2

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Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at:

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Requested From: United States
Date Requested: 11/3/2024