Products
Research Areas
COVID-19
Resources
Login
Quick Order
Cart Cart lightblue
Login
Registration enables users to use special features of this website, such as past
order histories, retained contact details for faster checkout, review submissions, and special promotions.


Fields marked with a * are required.

Login
Quick Order
Contact Us

Location


Corporate Headquarters

Vector Laboratories, Inc.
6737 Mowry Ave
Newark, CA 94560
United States

Telephone Numbers



Customer Service: (800) 227-6666 / (650) 697-3600


Contact Us



Additional Contact Details

Login
Registration enables users to use special features of this website, such as past
order histories, retained contact details for faster checkout, review submissions, and special promotions.


Fields marked with a * are required.

Login
Quick Order
Catalog Number Size Price
LS-C356224-100 100 µl (1 mg/ml) $315 
NFKB2 Antibody - Immunohistochemical analysis of NF-kappaB p100 (pS865) staining in human breast cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with hematoxylin and mounted with DPX.
NFKB2 Antibody - Immunofluorescent analysis of NF-kappaB p100 (pS865) staining in HepG2 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 deg C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue).
NFKB2 Antibody - Western blot analysis of NF-kappaB p100 (pS865) expression in HeLa (A); MCF7 (B) whole cell lysates.
NFKB2 Antibody - Immunohistochemical analysis of NF-kappaB p100 (pS865) staining in human breast cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with hematoxylin and mounted with DPX.
NFKB2 Antibody - Immunofluorescent analysis of NF-kappaB p100 (pS865) staining in HepG2 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 deg C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue).
NFKB2 Antibody - Western blot analysis of NF-kappaB p100 (pS865) expression in HeLa (A); MCF7 (B) whole cell lysates.
1 of 3
2 of 3
3 of 3

Polyclonal Rabbit anti‑Human NFKB2 Antibody (C‑Terminus, IHC, IF, WB) LS‑C356224

Polyclonal Rabbit anti‑Human NFKB2 Antibody (C‑Terminus, IHC, IF, WB) LS‑C356224

Antibody:
NFKB2 Rabbit anti-Human Polyclonal (C-Terminus) Antibody
Application:
IHC, IHC-P, ICC, IF, WB, IP
Reactivity:
Human, Mouse, Rat, Bovine, Pig, Chicken
Format:
Unconjugated, Unmodified
Price
Catalog Number
$315
LS-C356224-100
Toll Free North America
(800) 227-6666
For Research Use Only

Overview

Antibody:
NFKB2 Rabbit anti-Human Polyclonal (C-Terminus) Antibody
Application:
IHC, IHC-P, ICC, IF, WB, IP
Reactivity:
Human, Mouse, Rat, Bovine, Pig, Chicken
Format:
Unconjugated, Unmodified

Specifications

Description
NFKB2 antibody LS-C356224 is an unconjugated rabbit polyclonal antibody to NFKB2 (C-Terminus) from human. It is reactive with human, mouse, rat and other species. Validated for ICC, IF, IHC, IP and WB.
Target
Human NFKB2
Synonyms
NFKB2 | DNA-binding factor KBF2 | LYT10 | Oncogene Lyt-10 | p52 | LYT-10 | NF-kB2 | p105 | H2TF1
Host
Rabbit
Reactivity
Human, Mouse, Rat, Bovine, Pig, Chicken (tested or 100% immunogen sequence identity)
Clonality
Polyclonal
Conjugations
Unconjugated
Purification
Immunoaffinity purified
Modifications
Unmodified
Immunogen
KLH-conjugated synthetic peptide encompassing a sequence within the C-Terminus of human NF-kappaB p100 (pS865).
Epitope
C-Terminus
Specificity
Recognizes endogenous levels of NF-kappaB p100 (pS865) protein.
Applications
  • IHC
  • IHC - Paraffin (1:100 - 1:200)
  • ICC (1:100 - 1:500)
  • Immunofluorescence
  • Western blot (1:500 - 1:1000)
  • Immunoprecipitation (1:10 - 1:100)
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Presentation
PBS, pH 7.3, 0.01% Sodium Azide, 30% Glycerol
Storage
Store at -20°C. Aliquot to avoid freeze/thaw cycles.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee
About NFKB2
Q00653 NM_002502 NP_002493.3

Publications (0)

Customer Reviews (0)


Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at:

Technical.Support@LSBio.com

Requested From: United States
Date Requested: 11/24/2024