Human NGF

Rabbit

Human (tested or 100% immunogen sequence identity)

IgG Monoclonal

R711

Unconjugated

Protein A purified

Preservative Free

Recombinant Human NGF / NGFB protein

Human NGF. Has cross-reactivity with Mouse ß-NGF in ELISA assay

• ELISA (2 µg/ml)
• Blocking (5 µg/ml)
• Neutralization (10 ng/ml)

Block: In a functional ELISA which immobilized recombinant Human ß-NGF at 2ug/ml (100 µL/well) in the plate, the Rabbit anti-Human ß-NGF Monoclonal Antibody can block the binding of 5 ug/ml of biotinylated Human NTRK1/Fc Chimera Protein to human ß-NGF, the EC50 is 3. 84 ug/ml. Neutralization: The neutralization activity of antibody is Measured by its ability to neutralize ß­NGF­induced proliferation in the TF­1 human erythroleukemic cell line. The Neutralization titer (IC50) is typically 0.048­0.19 µg/mL in the presence of 10 ng/mL Recombinant Human ß­NGF. Please Note: Optimal concentrations/dilutions should be determined by the end user.

0.2 µm filtered histidine and arginine buffer containing 120mM NaCl, 0.02% Tween 80, pH 6.0

Store at 2°C to 8°C for up to 1 month. Aliquot and store at -20°C to -80°C for up to 1 year. Avoid freeze/thaw cycles.

For research use only. Intended for use by laboratory professionals.

This antibody carries the LSBio 100% Guarantee.

Uniprot: P01138 NCBI: NM_002506 NP_002497.2