Human NGF

Mouse

Human (tested or 100% immunogen sequence identity)

IgG2b Monoclonal

Unconjugated

Protein G purified

Unmodified

E.coli derived recombinant Human beta-NGF.

Human beta-NGF

• Western blot (4 - 8 µg/ml)
• ELISA (2 - 4 µg/ml)

Neutralization: To yield one-half maximal inhibition [ND50] of the biological activity of Human beta-NGF (10 ng/ml), a concentration of 2.7-4 ug/ml of this antibody is required. ELISA: In a sandwich ELISA (assuming 100 ul/well), a concentration of 2-4 ug/ml of this antibody will detect at least 200 pg/ml of recombinant Human beta-NGF when used with biotinylated antigen affinity purified anti-Human beta-NGF (LS-C104735) as the detection antibody at a concentration of approximately 0.5-1 ug/ml. Western Blot: To detect Human beta-NGF by Western Blot analysis this antibody can be used at a concentration of 4-8 ug/ml. When used in conjunction with compatible secondary reagents, the detection limit for recombinant Human beta-NGF is 4-8 ng/lane, under reducing or non-reducing conditions.

Lyophilized from Sterile Solution PBS

Centrifuge vial prior to opening. Reconstitiute in sterile water to 1.0 mg/ml.

Store Lyophilized at room temperature up to 1 month; Reconstituted for up to 2 weeks at 2°C to 8°C. Aliquot and freeze at -20°C for long term storage. Avoid freeze/thaw cycles.

For research use only. Intended for use by laboratory professionals.

This antibody carries the LSBio 100% Guarantee.

Uniprot: P01138 NCBI: NM_002506 NP_002497.2