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Catalog Number Size Price
LS-C37725-100 100 µg $786 
Antibody

Monoclonal Mouse anti‑Rat NOS1 / nNOS Antibody (N‑Terminus, IHC, WB) LS‑C37725

Monoclonal Mouse anti‑Rat NOS1 / nNOS Antibody (N‑Terminus, IHC, WB) LS‑C37725

Antibody:
NOS1 / nNOS Mouse anti-Rat Monoclonal (N-Terminus) Antibody
Application:
IHC, IHC-P, WB, IP, ELISA
Reactivity:
Rat
Format:
Unconjugated, Unmodified
Other formats:
Price
Catalog Number
$786
LS-C37725-100
Toll Free North America
(800) 227-6666
For Research Use Only

Overview

Antibody:
NOS1 / nNOS Mouse anti-Rat Monoclonal (N-Terminus) Antibody
Application:
IHC, IHC-P, WB, IP, ELISA
Reactivity:
Rat
Format:
Unconjugated, Unmodified
Other formats:

Specifications

Description
NNOS antibody LS-C37725 is an unconjugated mouse monoclonal antibody to rat nNOS (NOS1) (N-Terminus). Validated for ELISA, IHC, IP and WB.
Target
Rat NOS1 / nNOS
Synonyms
NOS1 | BNOS | Constitutive NOS | IHPS1 | NNOS | NC-NOS | Nitric oxide synthase, brain | N-NOS | Neuronal NOS | NOS | NOS type I | Nos, type i
Host
Mouse
Reactivity
Rat (tested or 100% immunogen sequence identity)
Clonality
IgG1,k Monoclonal
Conjugations
Unconjugated. Also available conjugated with APC, Biotin, FITC, HRP, PE.
Purification
Protein A purified
Modifications
Unmodified
Immunogen
Recombinant protein derived from the N-Terminus of rat nNOS protein.
Epitope
N-Terminus
Specificity
Specific for the ~160kD nNOS. Reactivity has been confirmed with rat brain tissue lysates. The nNOS target band at ~160kD was detected by immunoprecipitation and Western blots. Immunohistochemical tests using human, mouse, and rat brain tissue sections yielded positive and specific cytoplasmic staining in neurons and astrocytes. Based on amino acid sequence homology, the antibody is expected to recognize mouse (95%) and human (81%) nNOS.
Applications
  • IHC
  • IHC - Paraffin (2 µg/ml)
  • Western blot (1 µg/ml)
  • Immunoprecipitation
  • ELISA (0.1 - 1 µg/ml)
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Usage
Suitable for use in Western Blot, ELISA, Immunoprecipitation and Immunohistochemistry. Western Blot: 1 ug/ml. ELISA: 0.1-1 ug/ml. Immunoprecipitation: 7 ug/each. Immunohistochemistry (FFPE): 2 ug/ml.
Presentation
PBS, pH 7.4, 0.1% Sodium Azide
Storage
Store at 4°C or -20°C. Avoid freeze-thaw cycles.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee
About NOS1 / nNOS
P29475 NM_000620 NP_000611.1

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Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at:

Technical.Support@LSBio.com

Requested From: United States
Date Requested: 11/24/2024