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Catalog Number Size Price
LS-C16928-100 100 µl $634 
Antibody

Polyclonal Rabbit anti‑Human NOTCH1 Antibody (aa2487‑2501, IHC, WB) LS‑C16928

Polyclonal Rabbit anti‑Human NOTCH1 Antibody (aa2487‑2501, IHC, WB) LS‑C16928

Antibody:
NOTCH1 Rabbit anti-Human Polyclonal (aa2487-2501) Antibody
Application:
IHC, WB, ELISA
Reactivity:
Human
Format:
Unconjugated, Unmodified
Price
Catalog Number
$634
LS-C16928-100
Toll Free North America
(800) 227-6666
For Research Use Only

Overview

Antibody:
NOTCH1 Rabbit anti-Human Polyclonal (aa2487-2501) Antibody
Application:
IHC, WB, ELISA
Reactivity:
Human
Format:
Unconjugated, Unmodified

Specifications

Description
NOTCH1 antibody LS-C16928 is an unconjugated rabbit polyclonal antibody to human NOTCH1 (aa2487-2501). Validated for ELISA, IHC and WB. Cited in 2 publications.
Target
Human NOTCH1
Synonyms
NOTCH1 | Notch 1 | TAN1
Host
Rabbit
Reactivity
Human (tested or 100% immunogen sequence identity)
Clonality
Polyclonal
Conjugations
Unconjugated
Purification
Antiserum
Modifications
Unmodified
Immunogen
A synthetic peptide C-QHSYSSPVDNTPSHQ corresponding to amino acid residues 2488-2502 of human Notch 1, intracellular domain. A residue of cysteine was added to the N-Terminus end to facilitate coupling. Percent identity by BLAST analysis: Human, Gorilla (100%); Opossum, Chicken, Xenopus, Newt (93%); Platypus (87%); Trout, Stickleback, Pufferfish, Zebrafish (80%).
Epitope
aa2487-2501
Specificity
Recognizes human NOTCH 1. No cross-reactivity with NOTCH 2 or Mouse Notch.
Applications
  • IHC (1:500 - 1:2500)
  • Western blot (1:1000 - 1:5000)
  • ELISA (1:10000 - 1:50000)
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Usage
Suitable for use in, ELISA, Immunohistochemistry and Western Blot. ELISA: 1:10000-1:50000. Standard sandwich ELISA using IgG, H&L (HRP) Pab Gt xRb and ABTS as a substrate for 30 minutes at RT. Western Blot: 1:1000-1:5000. Immunohistochemistry: 1:500-1:2500.
Presentation
PBS, 0.1% sodium azide. No stabilizing proteins added.
Storage
Short term: store at 4°C. Long term: store at -20°C. Avoid freeze-thaw cycles.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee
About NOTCH1
P46531 NM_017617 NP_060087.3

LSBio Ratings

NOTCH1 Antibody (aa2487-2501) for IHC, WB/Western, ELISA LS-C16928 has an LSBio Rating of
Publications (4.1)

Publications (2)

Sonic hedgehog (Shh)/Gli modulates the spatial organization of neuroepithelial cell proliferation in the developing chick optic tectum. Rapacioli M, Botelho J, Cerda G, Duarte S, Elliot M, Palma V, Flores V. BMC neuroscience. 2012 13:117. (IHC, WB; Human)

Temporal-spatial correlation between angiogenesis and corticogenesis in the developing chick optic tectum. Alejandra Rodriguez Celin, Melina Rapacioli, Mariela Azul Gonzalez, Virginia Laura Ballarin, Sara Fiszer de Plazas, Juan José López-Costa, Vladimir Flores. PloS one. 2015 January;10:e0116343.

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Requested From: United States
Date Requested: 11/24/2024