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Catalog Number Size Price
LS-C672821-50 50 µg $294 
LS-C672821-100 100 µg $360 
OCLN / Occludin Antibody - Immunohistochemistry image at a dilution of 1:400 and staining in paraffin-embedded human colon cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0) . Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4 °C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.
OCLN / Occludin Antibody - Immunofluorescence staining of MCF-7 cells at a dilution of 1:133, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4 °C.The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG (H+L) .
OCLN / Occludin Antibody - Positive Western Blot detected in MCF-7 whole cell lysate. All lanes: OCLN antibody at 3.2 µg/ml Secondary Goat polyclonal to rabbit IgG at 1/50000 dilution. Predicted band size: 60, 53, 55, 32, 24, 9 KDa. Observed band size: 60 KDa
OCLN / Occludin Antibody - Immunohistochemistry image at a dilution of 1:400 and staining in paraffin-embedded human colon cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0) . Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4 °C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.
OCLN / Occludin Antibody - Immunofluorescence staining of MCF-7 cells at a dilution of 1:133, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4 °C.The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG (H+L) .
OCLN / Occludin Antibody - Positive Western Blot detected in MCF-7 whole cell lysate. All lanes: OCLN antibody at 3.2 µg/ml Secondary Goat polyclonal to rabbit IgG at 1/50000 dilution. Predicted band size: 60, 53, 55, 32, 24, 9 KDa. Observed band size: 60 KDa
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Polyclonal Rabbit anti‑Human OCLN / Occludin Antibody (IHC, IF, WB) LS‑C672821

Polyclonal Rabbit anti‑Human OCLN / Occludin Antibody (IHC, IF, WB) LS‑C672821

Antibody:
OCLN / Occludin Rabbit anti-Human Polyclonal Antibody
Application:
IHC, IHC-P, IF, WB, ELISA
Reactivity:
Human
Format:
Unconjugated, Unmodified
Other formats:
Price
Catalog Number
$294
LS-C672821-50
Toll Free North America
(800) 227-6666
For Research Use Only

Overview

Antibody:
OCLN / Occludin Rabbit anti-Human Polyclonal Antibody
Application:
IHC, IHC-P, IF, WB, ELISA
Reactivity:
Human
Format:
Unconjugated, Unmodified
Other formats:

Specifications

Description
Occludin antibody LS-C672821 is an unconjugated rabbit polyclonal antibody to human Occludin (OCLN). Validated for ELISA, IF, IHC and WB.
Target
Human OCLN / Occludin
Synonyms
OCLN | Occludin | BLCPMG
Host
Rabbit
Reactivity
Human (tested or 100% immunogen sequence identity)
Clonality
IgG Polyclonal
Conjugations
Unconjugated. Also available conjugated with FITC, Biotin, HRP.
Purification
Protein G purified
Modifications
Unmodified
Immunogen
Recombinant Human Occludin protein (285-430AA)
Applications
  • IHC
  • IHC - Paraffin
  • Immunofluorescence
  • Western blot
  • ELISA
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Presentation
0.01 M PBS, pH 7.4, 0.03% ProClin™ 300, 50% Glycerol
Storage
Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee
About OCLN / Occludin
Q16625 NM_002538 NP_002529.1

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Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at:

Technical.Support@LSBio.com

Requested From: United States
Date Requested: 11/22/2024