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Catalog Number Size Price
LS-C331587-20 20 µl $275 
LS-C331587-100 100 µl (0.168 mg/ml) $389 
Osteonectin / SPARC Antibody - Immunohistochemistry of paraffin-embedded human esophageal cancer using SPARC antibodyat dilution of 1:100 (40x lens).
Osteonectin / SPARC Antibody - Immunohistochemistry of paraffin-embedded mouse liver using SPARC antibodyat dilution of 1:100 (40x lens).
Osteonectin / SPARC Antibody - Immunohistochemistry of paraffin-embedded mouse kidney using SPARC antibodyat dilution of 1:100 (40x lens).
Osteonectin / SPARC Antibody - Immunohistochemistry of paraffin-embedded human liver cancer using SPARC antibodyat dilution of 1:100 (40x lens).
Osteonectin / SPARC Antibody - Immunohistochemistry of paraffin-embedded rat kidney using SPARC antibody at dilution of 1:100 (200x lens).
Osteonectin / SPARC Antibody - Immunohistochemistry of paraffin-embedded rat Intestine using SPARC antibodyat dilution of 1:100 (40x lens).
Osteonectin / SPARC Antibody - Immunohistochemistry of paraffin-embedded human lung cancer using SPARC antibodyat dilution of 1:100 (40x lens).
Osteonectin / SPARC Antibody - Western blot analysis of extracts of various cell lines, using SPARC antibody at 1:1000 dilution. The secondary antibody used was an HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates were loaded 25ug per lane and 3% nonfat dry milk in TBST was used for blocking.
Osteonectin / SPARC Antibody - Immunohistochemistry of paraffin-embedded human esophageal cancer using SPARC antibodyat dilution of 1:100 (40x lens).
Osteonectin / SPARC Antibody - Immunohistochemistry of paraffin-embedded mouse liver using SPARC antibodyat dilution of 1:100 (40x lens).
Osteonectin / SPARC Antibody - Immunohistochemistry of paraffin-embedded mouse kidney using SPARC antibodyat dilution of 1:100 (40x lens).
Osteonectin / SPARC Antibody - Immunohistochemistry of paraffin-embedded human liver cancer using SPARC antibodyat dilution of 1:100 (40x lens).
Osteonectin / SPARC Antibody - Immunohistochemistry of paraffin-embedded rat kidney using SPARC antibody at dilution of 1:100 (200x lens).
Osteonectin / SPARC Antibody - Immunohistochemistry of paraffin-embedded rat Intestine using SPARC antibodyat dilution of 1:100 (40x lens).
Osteonectin / SPARC Antibody - Immunohistochemistry of paraffin-embedded human lung cancer using SPARC antibodyat dilution of 1:100 (40x lens).
Osteonectin / SPARC Antibody - Western blot analysis of extracts of various cell lines, using SPARC antibody at 1:1000 dilution. The secondary antibody used was an HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates were loaded 25ug per lane and 3% nonfat dry milk in TBST was used for blocking.
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Polyclonal Rabbit anti‑Human Osteonectin / SPARC Antibody (IHC, WB) LS‑C331587

Polyclonal Rabbit anti‑Human Osteonectin / SPARC Antibody (IHC, WB) LS‑C331587

Antibody:
Osteonectin / SPARC Rabbit anti-Human Polyclonal Antibody
Application:
IHC, WB
Reactivity:
Human, Mouse, Rat
Format:
Unconjugated, Unmodified
Price
Catalog Number
$275
LS-C331587-20
Toll Free North America
(800) 227-6666
For Research Use Only

Overview

Antibody:
Osteonectin / SPARC Rabbit anti-Human Polyclonal Antibody
Application:
IHC, WB
Reactivity:
Human, Mouse, Rat
Format:
Unconjugated, Unmodified

Specifications

Description
SPARC antibody LS-C331587 is an unconjugated rabbit polyclonal antibody to SPARC (Osteonectin) from human. It is reactive with human, mouse and rat. Validated for IHC and WB.
Target
Human Osteonectin / SPARC
Synonyms
SPARC | Basement-membrane protein 40 | BM-40 | Cysteine-rich protein | ON | Osteonectin
Host
Rabbit
Reactivity
Human, Mouse, Rat (tested or 100% immunogen sequence identity)
Clonality
IgG Polyclonal
Conjugations
Unconjugated
Purification
Affinity purified
Modifications
Unmodified
Immunogen
Recombinant fusion protein containing a sequence corresponding to amino acids 18-303 of human SPARC (NP_003109.1). APQQEALPDETEVVEETVAEVTEVSVGANPVQVEVGEFDDGAEETEEEVVAENPCQNHHCKHGKVCELDENNTPMCVCQDPTSCPAPIGEFEKVCSNDNKTFDSSCHFFATKCTLEGTKKGHKLHLDYIGPCKYIPPCLDSELTEFPLRMRDWLKNVLVTLYERDEDNNLLTEKQKLRVKKIHENEKRLEAGDHPVELLARDFEKNYNMYIFPVHWQFGQLDQHPIDGYLSHTELAPLRAPLIPMEHCTTRFFETCDLDNDKYIALDEWAGCFGIKQKDIDKDLVI
Specificity
Human Osteonectin / SPARC
Applications
  • IHC (1:50 - 1:200)
  • Western blot (1:500 - 1:2000)
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Usage
The predicted MW is 34kDa, while the observed MW by Western blot was 35kDa.
Presentation
PBS, pH 7.3, 0.02% Sodium Azide, 50% Glycerol
Storage
Store at -20°C. Avoid freeze-thaw cycles.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee
About Osteonectin / SPARC
P09486 NM_003118 NP_003109.1

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Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at:

Technical.Support@LSBio.com

Requested From: United States
Date Requested: 11/3/2024