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Catalog Number Size Price
LS-C407983-10 10 µg $318 
LS-C407983-100 100 µg $470 
PDGFRA / PDGFR Alpha Antibody - PDGFRA antibody IHC-paraffin. IHC(P): Human Intestinal Cancer Tissue.
PDGFRA / PDGFR Alpha Antibody - Western blot analysis of PDGFRA using anti-PDGFRA antibody. Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human HT1080 whole cell lysates, Lane 2: human Caco-2 whole cell lysates, Lane 3: human U20S whole cell lysates, Lane 4: human PC-3 whole cell lysates, Lane 5: human 293T whole cell lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-PDGFRA antigen affinity purified polyclonal antibody at 0.5 µg/mL overnight at 4°C, then washed with TBS-0.1% Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for PDGFRA at approximately 123KD. The expected band size for PDGFRA is at 180KD.
PDGFRA / PDGFR Alpha Antibody - PDGFRA antibody Western blot. All lanes: Anti PDGFRA at 0.5 ug/ml. Lane 1: Rat Brain Tissue Lysate at 50 ug. Lane 2: HELA Whole Cell Lysate at 40 ug. Lane 3: NIH3T3 Whole Cell Lysate at 40 ug. Predicted band size: 180 kD. Observed band size: 180 kD.
PDGFRA / PDGFR Alpha Antibody - PDGFRA antibody IHC-paraffin. IHC(P): Human Intestinal Cancer Tissue.
PDGFRA / PDGFR Alpha Antibody - Western blot analysis of PDGFRA using anti-PDGFRA antibody. Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human HT1080 whole cell lysates, Lane 2: human Caco-2 whole cell lysates, Lane 3: human U20S whole cell lysates, Lane 4: human PC-3 whole cell lysates, Lane 5: human 293T whole cell lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-PDGFRA antigen affinity purified polyclonal antibody at 0.5 µg/mL overnight at 4°C, then washed with TBS-0.1% Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for PDGFRA at approximately 123KD. The expected band size for PDGFRA is at 180KD.
PDGFRA / PDGFR Alpha Antibody - PDGFRA antibody Western blot. All lanes: Anti PDGFRA at 0.5 ug/ml. Lane 1: Rat Brain Tissue Lysate at 50 ug. Lane 2: HELA Whole Cell Lysate at 40 ug. Lane 3: NIH3T3 Whole Cell Lysate at 40 ug. Predicted band size: 180 kD. Observed band size: 180 kD.
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Polyclonal Rabbit anti‑Human PDGFRA / PDGFR Alpha Antibody (aa968‑1002, IHC, WB) LS‑C407983

Polyclonal Rabbit anti‑Human PDGFRA / PDGFR Alpha Antibody (aa968‑1002, IHC, WB) LS‑C407983

Antibody:
PDGFRA / PDGFR Alpha Rabbit anti-Human Polyclonal (aa968-1002) Antibody
Application:
IHC, IHC-P, WB
Reactivity:
Human, Mouse, Rat, Bovine, Dog, Horse, Pig, Sheep
Format:
Unconjugated, Unmodified
Price
Catalog Number
$318
LS-C407983-10
Toll Free North America
(800) 227-6666
For Research Use Only

Overview

Antibody:
PDGFRA / PDGFR Alpha Rabbit anti-Human Polyclonal (aa968-1002) Antibody
Application:
IHC, IHC-P, WB
Reactivity:
Human, Mouse, Rat, Bovine, Dog, Horse, Pig, Sheep
Format:
Unconjugated, Unmodified

Specifications

Description
PDGFR Alpha antibody LS-C407983 is an unconjugated rabbit polyclonal antibody to PDGFR Alpha (PDGFRA) (aa968-1002) from human. It is reactive with human, mouse, rat and other species. Validated for IHC and WB.
Target
Human PDGFRA / PDGFR Alpha
Synonyms
PDGFRA | CD140A | CD140a antigen | PDGFalphaR | PDGFR2 | PDGFRalpha | AlphaPDGFR | RHEPDGFRA | PDGF receptor alpha | PDGFR-2 | PDGFRA/BCR fusion | APDGFR | Pdgf alpha-receptor | PDGF-R-alpha | PDGFR-alpha
Host
Rabbit
Reactivity
Human, Mouse, Rat, Bovine, Dog, Horse, Pig, Sheep (tested or 100% immunogen sequence identity)
Clonality
Polyclonal
Conjugations
Unconjugated
Purification
Immunogen affinity purified
Modifications
Unmodified
Immunogen
A synthetic peptide corresponding to a sequence at the C-Terminus of human PDGFRA (968-1002 aa DFLKSDHPAVARMRVDSDNAYIGVTYKNEEDKLKD), identical to the related mouse sequence, and different from the related rat sequence by one amino acid.
Epitope
aa968-1002
Specificity
Detected in platelets (at protein level). Widely expressed. Detected in brain, fibroblasts, smooth muscle, heart, and embryo. Expressed in primary and metastatic colon tumors and in normal colon tissue. .
Applications
  • IHC
  • IHC - Paraffin (0.5 - 1 µg/ml)
  • Western blot (0.1 - 0.5 µg/ml)
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Presentation
Lyophilized from 0.2mg Na2HPO4, 5mg BSA, 0.9mg NaCl, 0.05mg sodium azide.
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500µg/ml.
Storage
At -20°C for 1 year. After reconstitution, at 4°C for 1 month. It can also be aliquotted and stored frozen at -20°C for a longer time. Avoid freeze-thaw cycles.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee
About PDGFRA / PDGFR Alpha
P16234 NM_006206 NP_006197.1

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Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at:

Technical.Support@LSBio.com

Requested From: United States
Date Requested: 11/22/2024