Mouse PECAM-1 / CD31

Rat

Mouse (tested or 100% immunogen sequence identity)

Monoclonal

ER-MP12

Biotin

Affinity purified

Unmodified

Murine macrophage precursor cells

The antigen is a glutaraldehyde (0.05%) and paraformaldehyde (1%) resistant 140kD surface glycoprotein (reducing and non reducing conditions) identified as CD31. The antigen expression closely follows that of CD34. Antigen distribution: Isolated cells: The antigen is found on the surface of approximately 40% of freshly isolated bone marrow cells of adult mice, on the majority of immortalized macrophage precursor cell lines (M1, RMB-1, RMB-3) corresponding to CFU-GM cells. The antibody detects pluri- and multipotent stem cells and prothymocytes as well as particular lymphoid cells in bone marrow and peripheral lymphoid organs. Tissue sections: The antigen is detected within the lymphopoietic islands in the spleen of newborn (day 12) and in the bone marrow of adult mice. Capillary endothelial cells of adult mice also express the antigen. Specificity: Mouse: Major population of colony forming unit macrophage (CFU-M) precursor cells, subpopulation of pre CFU-M and monoblasts, precursor cells of granulocytes. Other: unknown

• IHC
• IHC - Paraffin (1:40)
• IHC - Frozen (1:1600)

The antigen is a glutaraldehyde (0.05%) and paraformaldehyde (1%) resistant. For IHC with paraffin embedded sections antigen retrieval with Proteinase K is recommended. The applications listed have been tested for the unconjugated form of this product. Other forms have not been tested.

Lyophilized from PBS, pH 7.2, 5 mg/ml BSA, 0.05% Kathon

Reconstitute with 0.5ml distilled water.

Store lyophilized at 4°C. Once reconstituted, aliquot and store at -20°C. Avoid freeze/thaw cycles. Store undiluted.

For research use only. Intended for use by laboratory professionals.

This antibody carries the LSBio 100% Guarantee.

Uniprot: P16284 NCBI: NM_000442 NP_000433.4