Human PLAP / Alkaline Phosphatase

Mouse

Human (tested or 100% immunogen sequence identity)

IgG1,k Monoclonal

ALPP/516

Unconjugated

Protein G purified

Unmodified. Also available Azide-free, BSA-free.

Recombinant full-length human ALPP protein.

Human PLAP / Alkaline Phosphatase

• IHC
• IHC - Frozen (1 - 2 µg/ml)
• Immunofluorescence (0.5 - 1 µg/ml)
• Flow Cytometry (0.5 - 1 µg/10E6 cells)

PLAP is a tissue specific, trophoblast-derived, 70kDa, glycosyl-phosphatidylinositol (GPI)-anchored, dimeric, Zn2+ metallo-glycoprotein that catalyzes the hydrolysis of phosphomonoesters into an inorganic phosphate and an alcohol. It is present in the placenta and serum of pregnant women and in high frequency in gynecological and testicular cancers and in lower frequency in other tumors. The three tissue-specific AP's in humans, PLAP, germ cell AP (GCAP) and intestinal AP, are 90-98% homologous. Non-tissue specific AP is found in kidney, liver and bone. This mAb binds equally well to all common allelic variants (S, F, FS and I) of PLAP and to some variants of AP from normal human testis. This mAb can be used as tracer antibody in ELISA to detect PLAP in serum of S, F, FS and I phenotypes. Optimal dilution of the placental Alkaline Phosphatase antibody should be determined by the researcher.

1X PBS, 0.1 mg/ml BSA, 0.05% sodium azide

Store at 2°C to 8°C.

For research use only. Intended for use by laboratory professionals.

This antibody carries the LSBio 100% Guarantee.

Uniprot: P05187 NCBI: NM_001632 NP_001623.3