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Antibodies > PRKCZ / PKC-Zeta > LS-C801151
 
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Catalog Number Size Price
LS-C801151-100 100 µl (1 mg/ml) $379 
LS-C801151-200 200 µl (1 mg/ml) $421 
PRKCZ / PKC-Zeta Antibody - 1:100 staining human brain tissue by IHC-P. The tissue was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The tissue was then blocked and incubated with the antibody for 1.5 hours at 22°C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.
PRKCZ / PKC-Zeta Antibody - Staining NIH-3T3 cells by IF/ICC. The samples were fixed with PFA and permeabilized in 0.1% Triton X-100, then blocked in 10% serum for 45 min at 25°C. The primary antibody was diluted at 1:200 and incubated with the sample for 1 hour at 37°C. An Alexa Fluor 594 conjugated goat anti-rabbit IgG (H+L) Ab, diluted at 1/600, was used as the secondary antibody.
PRKCZ / PKC-Zeta Antibody - Staining C6 cells treated with lambda phosphatase cells by IF/ICC. The samples were fixed with PFA and permeabilized in 0.1% saponin prior to blocking in 10% serum for 45 min at 37°C. The primary antibody was diluted 1/400 and incubated with the sample for 1 hour at 37°C. A Alexa Fluor 594 conjugated goat polyclonal to rabbit IgG (H+L), diluted 1/600 was used as secondary antibody.
PRKCZ / PKC-Zeta Antibody - Western blot analysis of PKC zeta phosphorylation expression in PMA treated NIH-3T3 whole cells lysates. The lane on the left is treated with the antigen-specific peptide.
PRKCZ / PKC-Zeta Antibody - Western blot analysis of PKC zeta phosphorylation expression in mouse liver tissue lysates. The lane on the right is treated with the antigen-specific peptide.
PRKCZ / PKC-Zeta Antibody - 1:100 staining human brain tissue by IHC-P. The tissue was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The tissue was then blocked and incubated with the antibody for 1.5 hours at 22°C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.
PRKCZ / PKC-Zeta Antibody - Staining NIH-3T3 cells by IF/ICC. The samples were fixed with PFA and permeabilized in 0.1% Triton X-100, then blocked in 10% serum for 45 min at 25°C. The primary antibody was diluted at 1:200 and incubated with the sample for 1 hour at 37°C. An Alexa Fluor 594 conjugated goat anti-rabbit IgG (H+L) Ab, diluted at 1/600, was used as the secondary antibody.
PRKCZ / PKC-Zeta Antibody - Staining C6 cells treated with lambda phosphatase cells by IF/ICC. The samples were fixed with PFA and permeabilized in 0.1% saponin prior to blocking in 10% serum for 45 min at 37°C. The primary antibody was diluted 1/400 and incubated with the sample for 1 hour at 37°C. A Alexa Fluor 594 conjugated goat polyclonal to rabbit IgG (H+L), diluted 1/600 was used as secondary antibody.
PRKCZ / PKC-Zeta Antibody - Western blot analysis of PKC zeta phosphorylation expression in PMA treated NIH-3T3 whole cells lysates. The lane on the left is treated with the antigen-specific peptide.
PRKCZ / PKC-Zeta Antibody - Western blot analysis of PKC zeta phosphorylation expression in mouse liver tissue lysates. The lane on the right is treated with the antigen-specific peptide.
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Polyclonal Rabbit anti‑Human PRKCZ / PKC‑Zeta Antibody (phospho‑Thr410, IHC, IF, WB) LS‑C801151

Polyclonal Rabbit anti‑Human PRKCZ / PKC‑Zeta Antibody (phospho‑Thr410, IHC, IF, WB) LS‑C801151

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Antibody:
PRKCZ / PKC-Zeta Rabbit anti-Human Polyclonal (pThr410) Antibody
Application:
IHC, IF, WB, Peptide-ELISA
Reactivity:
Human, Mouse, Rat
Format:
Unconjugated, Unmodified
Price
Catalog Number
$379
LS-C801151-100
Toll Free North America
(800) 227-6666
For Research Use Only
Specifications Validation Publications Reviews Featured Products Request SDS/MSDS

Overview

Antibody:
PRKCZ / PKC-Zeta Rabbit anti-Human Polyclonal (pThr410) Antibody
Application:
IHC, IF, WB, Peptide-ELISA
Reactivity:
Human, Mouse, Rat
Format:
Unconjugated, Unmodified

Specifications

Description
PKC-Zeta antibody LS-C801151 is an unconjugated rabbit polyclonal antibody to PKC-Zeta (PRKCZ) (pThr410) from human. It is reactive with human, mouse and rat. Validated for IF, IHC, Peptide-ELISA and WB.
Target
Human PRKCZ / PKC-Zeta
Synonyms
PRKCZ | PKC-ZETA | PKC2 | Pkcz | PKCzeta | Protein kinase c zeta | Protein kinase C zeta type | NPKC-zeta | PKC Zeta | Protein kinase C, zeta | Protein kinase Czeta
Host
Rabbit
Reactivity
Human, Mouse, Rat (tested or 100% immunogen sequence identity)
Clonality
IgG Polyclonal
Conjugations
Unconjugated
Purification
Affinity purification via sequential chromatography on phospho- and non-phospho-peptide affinity columns.
Modifications
Unmodified
Immunogen
A synthesized peptide derived from human PKC zeta around the phosphorylation site of Threonine 410.
Epitope
pThr410
Specificity
Phospho-PKC zeta (Thr410) Antibody detects endogenous levels of PKC zeta only when phosphorylated at Threonine 410.
Applications
  • IHC (1:50 - 1:200)
  • Immunofluorescence (1:100 - 1:500)
  • Western blot (1:500 - 1:2000)
  • Peptide Enzyme-Linked Immunosorbent Assay (1:20000 - 1:40000)
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Usage
For western blots: incubate membrane with diluted antibody overnight in 5% w/v milk , 1X TBS, 0.1% Tween-20 at 4°C with gentle shaking.
Presentation
PBS, pH 7.4, 0.02% Sodium Azide, 50% Glycerol
Storage
Upon receipt, store at -20°C. Avoid freeze-thaw cycles.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee
About PRKCZ / PKC-Zeta
Q05513 NM_002744 NP_002735.3

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Featured Products

PRKCZ / PKC-Zeta Antibody - Brain, Hypothalamus
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Species: Human
Applications: IHC, IHC - Paraffin, ELISA
PRKCZ / PKC-Zeta Antibody - Anti-PRKCZ / PKC-Zeta antibody IHC of human testis. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.
IHC‑plus™ PRKCZ / PKC‑Zeta Antibody (Cytoplasmic Domain) LS‑A8391
Species: Human
Applications: IHC, IHC - Paraffin, ELISA
PRKCZ / PKC-Zeta Antibody - Anti-PRKCZ / PKC-Zeta antibody IHC of human brain cerebellum. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody dilution 1:50.
IHC‑plus™ PRKCZ / PKC‑Zeta Antibody (Internal, clone EP1490(2)) LS‑B6294
Species: Human, Mouse, Rat
Applications: IHC, IHC - Paraffin, Western blot
PRKCZ / PKC-Zeta Antibody - IHC of p-PKC (T410) pAb in paraffin-embedded human lung carcinoma tissue.
PRKCZ / PKC‑Zeta Antibody (phospho‑Thr410) LS‑C177941
Species: Human, Mouse, Rat
Applications: IHC, IHC - Paraffin, Immunofluorescence, Western blot
PRKCZ / PKC-Zeta Antibody - Immunohistochemical analysis of PKC zeta staining in human breast cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with hematoxylin and mounted with DPX.
PRKCZ / PKC‑Zeta Antibody (C‑Terminus) LS‑C354069
Species: Human, Monkey, Mouse, Rat, Rabbit, Zebrafish
Applications: IHC, IHC - Paraffin, ICC, Immunofluorescence, Western blot, Immunoprecipitation
PRKCZ / PKC-Zeta Antibody - Mouse Prkcz Antibody western blot of mouse Neuro-2a cell line lysates (35 ug/lane). The Prkcz antibody detected the Prkcz protein (arrow).
PRKCZ / PKC‑Zeta Antibody (aa1‑30) LS‑C164554
Species: Mouse
Applications: Western blot
See all PRKCZ / PKC-Zeta Antibodies

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at:

Technical.Support@LSBio.com
Requested From: United States
Date Requested: 11/22/2024
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