Human PTGS2 / COX2 / COX-2

Rabbit

Human, Mouse, Rat (tested or 100% immunogen sequence identity)

Polyclonal

Unconjugated

Antiserum

Unmodified

This whole rabbit serum was prepared by repeated immunizations with a fusion protein corresponding to the carboxy-terminus of human Cox-2.

This antiserum is directed against human Cox-2 and is useful in determining its presence in western blotting and ELISA experiments. The antibody does not cross react with Cox-1. Cox-2 is involved in the response of cells to growth factors, tumor promoters, and cytokines that induce its expression. Given its role in synthesizing prostaglandins, Cox-2 is therefore of interest in studying immune response regulation. This antibody can detect Cox-2 in human, mouse and rat. Reactivity of this antibody with Cox-2 from other species is unknown.

• IHC
• IHC - Paraffin (1:500)
• Western blot (1:5000 - 1:10000)
• ELISA (1:10000 - 1:50000)

Immunohistochemistry: LS-B410 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B410 was determined to be 1:500.

Antiserum, 0.01% Sodium Azide

Store at 4°C or -20°C. Avoid freeze-thaw cycles.

For research use only. Intended for use by laboratory professionals.

This antibody carries the LSBio 100% Guarantee.

Uniprot: P35354 NCBI: NM_000963 NP_000954.1