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Catalog Number	Size	Price
LS-C413166-100	100 µl (1 mg/ml)	$321

PYCARD / ASC / TMS1 Antibody - Immunohistochemical analysis of ASC staining in human breast cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP polymer system. DAB was used as the chromogen. The section was then counterstained with hematoxylin and mounted with DPX.

PYCARD / ASC / TMS1 Antibody - Immunofluorescent analysis of ASC staining in HepG2 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 deg C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue).

PYCARD / ASC / TMS1 Antibody - Western blot analysis of ASC expression in HEK293T (A); Raw264.7 (B); mouse brain (C); rat brain (D) whole cell lysates.

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Polyclonal Rabbit anti‑Human PYCARD / ASC / TMS1 Antibody (N‑Terminus, IHC, IF, WB) LS‑C413166

Name: Anti-PYCARD / ASC / TMS1 Antibody | Rabbit anti-Human Polyclonal | LSBio
Brand: Lifespan BioSciences
SKU: LS-C413166-100
Price: 321.0000 USD
Availability: InStock

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Antibody:

PYCARD / ASC / TMS1 Rabbit anti-Human Polyclonal (N-Terminus) Antibody

Application:

IHC, IHC-P, ICC, IF, WB

Reactivity:

Human, Mouse, Rat

Format:

Unconjugated, Unmodified

$321

LS-C413166-100

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For Research Use Only

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Overview

Antibody:

PYCARD / ASC / TMS1 Rabbit anti-Human Polyclonal (N-Terminus) Antibody

Application:

IHC, IHC-P, ICC, IF, WB

Reactivity:

Human, Mouse, Rat

Format:

Unconjugated, Unmodified

Specifications

Description

TMS1 antibody LS-C413166 is an unconjugated rabbit polyclonal antibody to TMS1 (PYCARD / ASC) (N-Terminus) from human. It is reactive with human, mouse and rat. Validated for ICC, IF, IHC and WB.

Target

Human PYCARD / ASC / TMS1

Synonyms

PYCARD | CARD5 | HASC | ASC | TMS | TMS-1 | PYD and CARD domain containing | TMS1

See All PYCARD / ASC / TMS1 Antibodies

Host

Rabbit

Reactivity

Human, Mouse, Rat (tested or 100% immunogen sequence identity)

Clonality

Polyclonal

Conjugations

Unconjugated

Purification

Immunoaffinity purified

Modifications

Unmodified

Immunogen

KLH-conjugated synthetic peptide encompassing a sequence within the N-Terminus of human ASC.

Epitope

N-Terminus

Specificity

Recognizes endogenous levels of ASC protein.

Applications

IHC
IHC - Paraffin (1:100 - 1:200)
ICC (1:100 - 1:500)
Immunofluorescence (1:100 - 1:500)
Western blot (1:500 - 1:1000)

Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.

Presentation

PBS, pH 7.3, 0.01% Sodium Azide, 30% Glycerol

Storage

Aliquot and store at -20°C for 1 year. Avoid freeze/thaw cycles.

Restrictions

For research use only. Intended for use by laboratory professionals.

Guarantee

This antibody carries the LSBio 100% Guarantee.

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About PYCARD / ASC / TMS1

Uniprot: Q9ULZ3 NCBI: NM_013258 NP_037390.2