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Antibodies > RELA / NFKB p65 > LS-C800713
 
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Catalog Number Size Price
LS-C800713-100 100 µl (1 mg/ml) $379 
LS-C800713-200 200 µl (1 mg/ml) $421 
RELA / NFKB p65 Antibody - 1:100 staining mouse testis tissue by IHC-P. The tissue was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The tissue was then blocked and incubated with the antibody for 1.5 hours at 22°C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.
RELA / NFKB p65 Antibody - 1:100 staining human liver tissue by IHC-P. The tissue was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The tissue was then blocked and incubated with the antibody for 1.5 hours at 22°C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.
RELA / NFKB p65 Antibody - 1:100 staining rat ovarian tissue by IHC-P. The tissue was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The tissue was then blocked and incubated with the antibody for 1.5 hours at 22°C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.
RELA / NFKB p65 Antibody - 1:100 staining rat lung tissue by IHC-P. The tissue was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The tissue was then blocked and incubated with the antibody for 1.5 hours at 22°C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.
RELA / NFKB p65 Antibody - 1:100 staining mouse gastric tissue by IHC-P. The tissue was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The tissue was then blocked and incubated with the antibody for 1.5 hours at 22°C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.
RELA / NFKB p65 Antibody - 1:100 staining human brain tissue by IHC-P. The tissue was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The tissue was then blocked and incubated with the antibody for 1.5 hours at 22°C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.
RELA / NFKB p65 Antibody - 1:100 staining mouse kidney tissue by IHC-P. The tissue was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The tissue was then blocked and incubated with the antibody for 1.5 hours at 22°C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.
RELA / NFKB p65 Antibody - 1:100 staining rat uterine tissue by IHC-P. The tissue was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The tissue was then blocked and incubated with the antibody for 1.5 hours at 22°C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.
RELA / NFKB p65 Antibody - 1:100 staining human heart tissue by IHC-P. The tissue was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The tissue was then blocked and incubated with the antibody for 1.5 hours at 22°C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.
RELA / NFKB p65 Antibody - Staining HeLa cells by IF/ICC. The samples were fixed with PFA and permeabilized in 0.1% Triton X-100, then blocked in 10% serum for 45 min at 25°C. The primary antibody was diluted at 1:200 and incubated with the sample for 1 hour at 37°C. An Alexa Fluor 594 conjugated goat anti-rabbit IgG (H+L) Ab, diluted at 1/600, was used as the secondary antibody.
RELA / NFKB p65 Antibody - Western blot analysis of NF-kappaB p65 phosphorylation expression in IL-1 treated RAW264.7 whole cells lysates. The lane on the left is treated with the antigen-specific peptide.
RELA / NFKB p65 Antibody - 1:100 staining mouse testis tissue by IHC-P. The tissue was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The tissue was then blocked and incubated with the antibody for 1.5 hours at 22°C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.
RELA / NFKB p65 Antibody - 1:100 staining human liver tissue by IHC-P. The tissue was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The tissue was then blocked and incubated with the antibody for 1.5 hours at 22°C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.
RELA / NFKB p65 Antibody - 1:100 staining rat ovarian tissue by IHC-P. The tissue was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The tissue was then blocked and incubated with the antibody for 1.5 hours at 22°C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.
RELA / NFKB p65 Antibody - 1:100 staining rat lung tissue by IHC-P. The tissue was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The tissue was then blocked and incubated with the antibody for 1.5 hours at 22°C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.
RELA / NFKB p65 Antibody - 1:100 staining mouse gastric tissue by IHC-P. The tissue was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The tissue was then blocked and incubated with the antibody for 1.5 hours at 22°C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.
RELA / NFKB p65 Antibody - 1:100 staining human brain tissue by IHC-P. The tissue was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The tissue was then blocked and incubated with the antibody for 1.5 hours at 22°C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.
RELA / NFKB p65 Antibody - 1:100 staining mouse kidney tissue by IHC-P. The tissue was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The tissue was then blocked and incubated with the antibody for 1.5 hours at 22°C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.
RELA / NFKB p65 Antibody - 1:100 staining rat uterine tissue by IHC-P. The tissue was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The tissue was then blocked and incubated with the antibody for 1.5 hours at 22°C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.
RELA / NFKB p65 Antibody - 1:100 staining human heart tissue by IHC-P. The tissue was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The tissue was then blocked and incubated with the antibody for 1.5 hours at 22°C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.
RELA / NFKB p65 Antibody - Staining HeLa cells by IF/ICC. The samples were fixed with PFA and permeabilized in 0.1% Triton X-100, then blocked in 10% serum for 45 min at 25°C. The primary antibody was diluted at 1:200 and incubated with the sample for 1 hour at 37°C. An Alexa Fluor 594 conjugated goat anti-rabbit IgG (H+L) Ab, diluted at 1/600, was used as the secondary antibody.
RELA / NFKB p65 Antibody - Western blot analysis of NF-kappaB p65 phosphorylation expression in IL-1 treated RAW264.7 whole cells lysates. The lane on the left is treated with the antigen-specific peptide.
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Polyclonal Rabbit anti‑Human RELA / NFKB p65 Antibody (phospho‑Ser536, IHC, IF, WB) LS‑C800713

Polyclonal Rabbit anti‑Human RELA / NFKB p65 Antibody (phospho‑Ser536, IHC, IF, WB) LS‑C800713

Submit Review LSBio Guarantee
Antibody:
RELA / NFKB p65 Rabbit anti-Human Polyclonal (pSer536) Antibody
Application:
IHC, IF, WB, IP, Peptide-ELISA
Reactivity:
Human, Mouse, Rat
Format:
Unconjugated, Unmodified
Price
Catalog Number
$379
LS-C800713-100
Toll Free North America
(800) 227-6666
For Research Use Only
Specifications Validation Publications Reviews Featured Products Request SDS/MSDS

Overview

Antibody:
RELA / NFKB p65 Rabbit anti-Human Polyclonal (pSer536) Antibody
Application:
IHC, IF, WB, IP, Peptide-ELISA
Reactivity:
Human, Mouse, Rat
Format:
Unconjugated, Unmodified

Specifications

Description
NFKB p65 antibody LS-C800713 is an unconjugated rabbit polyclonal antibody to NFKB p65 (RELA) (pSer536) from human. It is reactive with human, mouse and rat. Validated for IF, IHC, IP, Peptide-ELISA and WB.
Target
Human RELA / NFKB p65
Synonyms
RELA | NF-kappa-B p65delta3 | NF-kappaB | NFKB3 | p65 | Transcription factor p65 | NF kappaB | NFKB
Host
Rabbit
Reactivity
Human, Mouse, Rat (tested or 100% immunogen sequence identity)
Clonality
IgG Polyclonal
Conjugations
Unconjugated
Purification
Affinity purification via sequential chromatography on phospho- and non-phospho-peptide affinity columns.
Modifications
Unmodified
Immunogen
A synthesized peptide derived from human NF- kappaB p65 around the phosphorylation site of Serine 536.
Epitope
pSer536
Specificity
Phospho-NF- kappaB p65 (Ser536) Antibody detects endogenous levels of NF- kappaB p65 only when phosphorylated at Serine 536.
Applications
  • IHC (1:50 - 1:500)
  • Immunofluorescence
  • Western blot (1:500 - 1:2000)
  • Immunoprecipitation (1:100 - 1:500)
  • Peptide Enzyme-Linked Immunosorbent Assay (1:20000 - 1:40000)
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Usage
For western blots: incubate membrane with diluted antibody overnight in 5% w/v milk , 1X TBS, 0.1% Tween-20 at 4°C with gentle shaking.
Presentation
PBS, pH 7.4, 150 mM NaCl, Sodium Azide, Glycerol
Storage
Upon receipt, store at -20°C. Avoid freeze-thaw cycles.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee
About RELA / NFKB p65
Q04206 NM_021975 NP_068810.3

Publications (0)

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RELA / NFKB p65 Antibody - Anti-RELA antibody IHC of human brain, cortex. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody dilution 1:500.
IHC‑plus™ RELA / NFKB p65 Antibody (phospho‑Ser529) LS‑B652
Species: Human
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RELA / NFKB p65 Antibody - Anti-RELA antibody IHC of human kidney. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.
IHC‑plus™ RELA / NFKB p65 Antibody (phospho‑Ser536) LS‑B53
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RELA / NFKB p65 Antibody - Western blot of Acetyl-NFkappaB-p65 (K122) antibody
RELA / NFKB p65 Antibody (Acetyl‑Lys122) LS‑C387648
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Product
RELA / NFKB p65 Antibody (aa297‑308) LS‑C9296
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RELA / NFKB p65 Antibody - Anti-RELA antibody IHC of human kidney. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody dilution 1:500.
IHC‑plus™ RELA / NFKB p65 Antibody (C‑Terminus) LS‑B653
Species: Human, Mouse, Rat
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RELA / NFKB p65 Antibody - Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue.
RELA / NFKB p65 Antibody (phospho‑Ser276) LS‑C335799
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Applications: IHC, Immunofluorescence, Western blot
See all RELA / NFKB p65 Antibodies

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at:

Technical.Support@LSBio.com
Requested From: United States
Date Requested: 11/23/2024
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6737 Mowry Ave
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United States

Tel: (800) 227-6666 / (650) 697-3600

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