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| Catalog Number | Size | Price |
|---|---|---|
| LS-C801138-100 | 100 µl (1 mg/ml) | $379 |
| LS-C801138-200 | 200 µl (1 mg/ml) | $421 |
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Polyclonal Rabbit anti‑Human RELA / NFKB p65 Antibody (phospho‑Thr254, IHC, IF, WB) LS‑C801138
Polyclonal Rabbit anti‑Human RELA / NFKB p65 Antibody (phospho‑Thr254, IHC, IF, WB) LS‑C801138
Antibody:
RELA / NFKB p65 Rabbit anti-Human Polyclonal (pThr254) Antibody
Application:
IHC, IF, WB, IP, Peptide-ELISA
Reactivity:
Human, Mouse, Rat
Format:
Unconjugated, Unmodified
Toll Free North America
(800) 227-6666
(800) 227-6666For Research Use Only
Overview
Antibody:
RELA / NFKB p65 Rabbit anti-Human Polyclonal (pThr254) Antibody
Application:
IHC, IF, WB, IP, Peptide-ELISA
Reactivity:
Human, Mouse, Rat
Format:
Unconjugated, Unmodified
Specifications
Description
NFKB p65 antibody LS-C801138 is an unconjugated rabbit polyclonal antibody to NFKB p65 (RELA) (pThr254) from human. It is reactive with human, mouse and rat. Validated for IF, IHC, IP, Peptide-ELISA and WB.
Target
Human RELA / NFKB p65
Synonyms
RELA | NF-kappa-B p65delta3 | NF-kappaB | NFKB3 | p65 | Transcription factor p65 | NF kappaB | NFKB
Host
Rabbit
Reactivity
Human, Mouse, Rat
(tested or 100% immunogen sequence identity)
Clonality
IgG
Polyclonal
Conjugations
Unconjugated
Purification
Affinity purification via sequential chromatography on phospho- and non-phospho-peptide affinity columns.
Modifications
Unmodified
Immunogen
A synthesized peptide derived from human NF- kappaB p65 around the phosphorylation site of Threonine 254.
Epitope
pThr254
Specificity
Phospho-NF- kappaB p65 (Thr254) Antibody detects endogenous levels of NF- kappaB p65 only when phosphorylated at Threonine 254.
Applications
- IHC (1:50 - 1:200)
- Immunofluorescence (1:100 - 1:500)
- Western blot (1:500 - 1:2000)
- Immunoprecipitation
- Peptide Enzyme-Linked Immunosorbent Assay (1:20000 - 1:40000)
|
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents
ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
|
Usage
For western blots: incubate membrane with diluted antibody overnight in 5% w/v milk , 1X TBS, 0.1% Tween-20 at 4°C with gentle shaking.
Presentation
PBS, pH 7.4, 0.02% Sodium Azide, 50% Glycerol
Storage
Upon receipt, store at -20°C. Avoid freeze-thaw cycles.
Restrictions
For research use only. Intended for use by laboratory professionals.
About RELA / NFKB p65
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Featured Products
Species:
Human
Applications:
IHC, IHC - Paraffin, Western blot, ELISA
Species:
Human
Applications:
IHC, IHC - Paraffin, Western blot, ELISA
Species:
Human, Mouse, Rat
Applications:
Western blot, ELISA
Species:
Human, Monkey, Mouse, Rat, Bat, Bovine, Dog, Hamster, Horse
Applications:
IHC, Immunofluorescence, Western blot, ELISA
Species:
Human, Mouse, Rat
Applications:
IHC, IHC - Paraffin, Western blot, ELISA, Chromatin Immunoprecipitation, Gel shift
Species:
Human
Applications:
IHC, Immunofluorescence, Western blot
Request SDS/MSDS
To request an SDS/MSDS form for this product, please contact our Technical Support department at:
Technical.Support@LSBio.com
Requested From: United States
Date Requested: 11/27/2024
Date Requested: 11/27/2024
