Rat SLC6A3 / Dopamine Transporter

Rabbit

Rat, Mouse (tested or 100% immunogen sequence identity)

IgG Polyclonal

Unconjugated

Immunoaffinity purified

Unmodified

An 18 amino acid peptide sequence near the NH2-terminus of rat brain dopamine transporter coupled to KLH (1). This region is presumably the cytoplasmic domain.

N-Terminus

Recognizes Dopamine transporter (DAT). Species cross-reactivity: Rat and mouse. Weakly reacts with human and canine.

• IHC
• IHC - Frozen
• Western blot (1 - 10 µg/ml)
• Immunoprecipitation
• ELISA (0.5 - 1 µg/ml)

Suitable for use in Western Blot, Immunoprecipitation, Immunohistochemistry, and ELISA. Western Blot: 1-10 ug/ml using ECL. An antibody raised against a similar peptide has been shown to react with a broad 80kD band in rat striatum and not cerebellum. Rat DAT contains various carbohydrate moieties and neuraminidase and N-Glycanase treatment reduces its molecular weight to about 55kD. Immunoprecipitation: 2-10 ug. Immunohistochemistry: 2-10 ug/ml on frozen sections of tissues fixed with 4% paraformaldehyde. ELISA: 0.5-1.0 ug/ml using 1 ug/ml of DAT control peptide per well.

PBS, pH 7.4, 0.05% Sodium Azide, 0.1% BSA

Short term: 4°C; Long term: Add glycerol (40-50%) -20°C.

For research use only. Intended for use by laboratory professionals.

This antibody carries the LSBio 100% Guarantee.

Uniprot: Q01959 NCBI: NM_001044 NP_001035.1