LSBio logo with text
Products
Research Areas
COVID-19
Resources
Contact Us
Login
Quick Order
Cart Cart lightblue
Login
Registration enables users to use special features of this website, such as past
order histories, retained contact details for faster checkout, review submissions, and special promotions.


Fields marked with a * are required.

Login
Quick Order
Cart
How To Buy
Contact Us

Location

Corporate Headquarters

Vector Laboratories, Inc.
6737 Mowry Ave
Newark, CA 94560
United States

Telephone Numbers


Customer Service: (800) 227-6666 / (650) 697-3600


Contact Us


Additional Contact Details

Distributors
Login
Registration enables users to use special features of this website, such as past
order histories, retained contact details for faster checkout, review submissions, and special promotions.


Fields marked with a * are required.

Login
Quick Order
Antibodies > SMAD1 > LS-C801200
 
Print Page  
Catalog Number Size Price
LS-C801200-100 100 µl (1 mg/ml) $379 
LS-C801200-200 200 µl (1 mg/ml) $421 
SMAD1 Antibody - 1/100 staining human breast carcinoma tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the antibody for 1.5 hours at 22°C. An HRP conjugated goat anti-rabbit antibody was used as the secondary antibody.
SMAD1 Antibody - 1:100 staining mouse muscle tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the antibody for 1.5 hours at 22°C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.
SMAD1 Antibody - Staining HeLa cells by IF/ICC. The samples were fixed with PFA and permeabilized in 0.1% Triton X-100, then blocked in 10% serum for 45 min at 25°C. The primary antibody was diluted at 1:200 and incubated with the sample for 1 hour at 37°C. An Alexa Fluor 594 conjugated goat anti-rabbit IgG (H+L) Ab, diluted at 1/600, was used as the secondary antibody.
SMAD1 Antibody - Western blot analysis of Smad1 phosphorylation expression in mouse muscle tissue lysates. The lane on the left is treated with the antigen-specific peptide.
SMAD1 Antibody - Western blot analysis of Phospho-Smad1 (Ser187) expression in various lysates
SMAD1 Antibody - 1/100 staining human breast carcinoma tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the antibody for 1.5 hours at 22°C. An HRP conjugated goat anti-rabbit antibody was used as the secondary antibody.
SMAD1 Antibody - 1:100 staining mouse muscle tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the antibody for 1.5 hours at 22°C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.
SMAD1 Antibody - Staining HeLa cells by IF/ICC. The samples were fixed with PFA and permeabilized in 0.1% Triton X-100, then blocked in 10% serum for 45 min at 25°C. The primary antibody was diluted at 1:200 and incubated with the sample for 1 hour at 37°C. An Alexa Fluor 594 conjugated goat anti-rabbit IgG (H+L) Ab, diluted at 1/600, was used as the secondary antibody.
SMAD1 Antibody - Western blot analysis of Smad1 phosphorylation expression in mouse muscle tissue lysates. The lane on the left is treated with the antigen-specific peptide.
SMAD1 Antibody - Western blot analysis of Phospho-Smad1 (Ser187) expression in various lysates
1 of 5
2 of 5
3 of 5
4 of 5
5 of 5

Polyclonal Rabbit anti‑Human SMAD1 Antibody (phospho‑Ser187, IHC, IF, WB) LS‑C801200

Polyclonal Rabbit anti‑Human SMAD1 Antibody (phospho‑Ser187, IHC, IF, WB) LS‑C801200

Submit Review LSBio Guarantee
Antibody:
SMAD1 Rabbit anti-Human Polyclonal (pSer187) Antibody
Application:
IHC, IF, WB, Peptide-ELISA
Reactivity:
Human, Mouse
Format:
Unconjugated, Unmodified
Price
Catalog Number
$379
LS-C801200-100
Toll Free North America
(800) 227-6666
For Research Use Only
Specifications Validation Publications Reviews Featured Products Request SDS/MSDS

Overview

Antibody:
SMAD1 Rabbit anti-Human Polyclonal (pSer187) Antibody
Application:
IHC, IF, WB, Peptide-ELISA
Reactivity:
Human, Mouse
Format:
Unconjugated, Unmodified

Specifications

Description
SMAD1 antibody LS-C801200 is an unconjugated rabbit polyclonal antibody to SMAD1 (pSer187) from human. It is reactive with human and mouse. Validated for IF, IHC, Peptide-ELISA and WB.
Target
Human SMAD1
Synonyms
SMAD1 | BSP1 | BSP-1 | JV4-1 | MADH1 | Mothers against DPP homolog 1 | JV41 | SMAD family member 1 | TGF-beta signaling protein 1 | MADR1 | HSMAD1 | MAD homolog 1 | Mad-related protein 1 | SMAD 1
Host
Rabbit
Reactivity
Human, Mouse (tested or 100% immunogen sequence identity)
Clonality
IgG Polyclonal
Conjugations
Unconjugated
Purification
Affinity purification via sequential chromatography on phospho- and non-phospho-peptide affinity columns.
Modifications
Unmodified
Immunogen
A synthesized peptide derived from human Smad1 around the phosphorylation site of Serine 187.
Epitope
pSer187
Specificity
Phospho-Smad1 (Ser187) Antibody detects endogenous levels of Smad1 only when phosphorylated at Serine 187.
Applications
  • IHC (1:50 - 1:200)
  • Immunofluorescence (1:100 - 1:500)
  • Western blot (1:500 - 1:2000)
  • Peptide Enzyme-Linked Immunosorbent Assay (1:20000 - 1:40000)
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Usage
For western blots: incubate membrane with diluted antibody overnight in 5% w/v milk , 1X TBS, 0.1% Tween-20 at 4°C with gentle shaking.
Presentation
PBS, pH 7.4, 0.02% Sodium Azide, 50% Glycerol
Storage
Upon receipt, store at -20°C. Avoid freeze-thaw cycles.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee
About SMAD1
Q15797 NM_005900 NP_005891.1

Publications (0)

Submit a Publication for LS-C801200

Customer Reviews (0)

Submit a review for LS-C801200

Featured Products

Product
SMAD1 Antibody LS‑C75853
Species: Human, Mouse, Rat
Applications: ICC, Western blot, Immunoprecipitation, Flow Cytometry
SMAD1 Antibody - Anti-SMAD1 Antibody - Western Blot. Western blot of Affinity Purified anti-SMAD1 antibody shows detection of endogenous SMAD1 in whole cell lysates from human hepatoma (HEPG2, lanes 1-4) and keratinocyte (HaCaT, lanes 5-8) derived cell lines treated with PBS, BMP2, EGF, or NaCl for 1 h at 37?C before harvest. Each lane contains approximately 15 ug of lysate. Primary antibody was used at a 1:500 dilution in 1% BLOTTO (B501-0500) and reacted for 1 hour at room temperature. Anti-beta actin (p/n LS-B323) staining was used as a loading control. The membrane was washed and reacted with a 1:3000 dilution HRP-conjugated a-Rabbit IgG (p/n LS-C60865) for 1 hour at room temperature. Detection was by ECL. Personal communication, Xin-Hua Feng, Baylor College of Medicine, Houston, TX.
SMAD1 Antibody LS‑C154249
Species: Human, Mouse, Rat, Dog
Applications: Western blot, Immunoprecipitation, ELISA
SMAD1 Antibody - Immunofluorescence of monoclonal antibody to SMAD1 on HeLa cell. [antibody concentration 10 ug/ml]
SMAD1 Antibody (clone 1D3) LS‑C133298
Species: Human
Applications: Immunofluorescence, Western blot, ELISA
SMAD1 Antibody - SMAD1 antibody SMAD1(SMAD family member 1) Antibody was used in IHC to stain formalin-fixed, paraffin-embedded human skin. This image was taken for the unconjugated form of this product. Other forms have not been tested.
SMAD1 Antibody (N‑Terminus) LS‑C80224
Species: Human, Mouse
Applications: IHC, IHC - Paraffin, Western blot
SMAD1 Antibody - A: Marker, B: Jurkat Cell Lysate. This image was taken for the unconjugated form of this product. Other forms have not been tested.
SMAD1 Antibody (C‑Terminus) LS‑C31108
Species: Human
Applications: Western blot
SMAD1 Antibody - Immunohistochemical analysis of SMAD1 (pS187) staining in human breast cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with hematoxylin and mounted with DPX.
SMAD1 Antibody (phospho‑Ser187) LS‑C352485
Species: Human, Mouse, Rat, Sheep, Chicken
Applications: IHC, IHC - Paraffin, Western blot, Immunoprecipitation
See all SMAD1 Antibodies

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at:

Technical.Support@LSBio.com
Requested From: United States
Date Requested: 2/11/2025
Family of Companies
Site Links Home All Antibodies How To Buy All Proteins Distributors All ELISA & Assay Kits Reviews PathPlus™ Antibodies
Follow LSBio View Cart Register or Login
Copyright © 2024 LifeSpan BioSciences, Inc - Privacy Policy
Contact Us

Vector Laboratories, Inc.
6737 Mowry Ave
Newark, CA 94560
United States

Tel: (800) 227-6666 / (650) 697-3600

Orders:
customerservice@vectorlabs.com

For technical assistance:
Technical.Support@LSBio.com