Human SOX2

Rabbit

Human (tested or 100% immunogen sequence identity)

Human, Bovine, Dog, Frog (at least 90% immunogen sequence identity)

IgG Monoclonal

SP76

Unconjugated

Protein A/G purified

Unmodified

Synthetic peptide corresponding to N-Terminus of human SOX-2 protein.

N-Terminus

Human SOX2

• IHC
• IHC - Paraffin (1:100)
• Western blot (1:25)

IMMUNOHISTOCHEMISTRY: Formalin-fixed, paraffin-embedded tissues are suitable for use with this primary antibody. Deparaffinization: Deparaffinize slides using xylene or xylene alternative and graded alcohols. Antibody Dilution: If using the concentrate format of this product, dilute the antibody 1:100. Antigen Retrieval: Boil tissue section in 10 mM citrate buffer, pH 6.0 for 10 min followed by cooling at room temperature for 20 min. Primary Antibody Incubation: Incubate for 30 minutes at room temperature. Slide Washing: Slides must be washed in between steps. Rinse slides with PBS/0.05% Tween. IHC Positive control: Newborn brain. WESTERN BLOT: Recommended starting protocol: Dilute the antibody 1:25. Incubate for 1 hour at room temperature. The dilution is an estimate. actual results may differ because of variability in methods and protocols. Positive Control: MCF7 cell lysate.

10 mM PBS, pH 7.4, 0.1% Sodium Azide, BSA

Aliquot and store at -20°C or -80°C. Avoid freeze-thaw cycles.

For research use only. Intended for use by laboratory professionals.

This antibody carries the LSBio 100% Guarantee.

Uniprot: P48431 NCBI: L07335 AAA35997.1