Human SYP / Synaptophysin

Mouse

Human (tested or 100% immunogen sequence identity)

IgG1 Monoclonal

SY38

Unconjugated

Ascites

Concentrated

The antibody is specific for Synaptophysin. The specificity was ascertained by immunoblotting and immunohistochemistry. The antibody predominantly reacts with a 38 kD transmembrane glycoprotein from synaptic vesicles. Antigen was isolated from presynaptic vesicles. Antigen was isolated from presynaptic vesicles. Antigen location, cytoplasm. Cross-reactivity with rat, mouse and cow.

• IHC
• IHC - Paraffin
• IHC - Frozen

Staining of normal neuroendocrine and neoplastic neuroendocrine cells in tissue sections. The antibody recognizes presynaptic vesicles of neurons of brain, spinal cord, retina neuromuscular junctions, small vesicles of adrenal medulla and pancreatic islets. The antibody can be used for the characterization of neuroendocrine tumors. The antibody can be used on frozen sections and paraffin embedded tissue, proteolytic or T. U. F. pretreatment is not necessary but may, depending on the length of the fixation period, enhance the staining. As positive control adrenal pheochromocytoma and pancreatic islet cell neoplasm are recommended. A dilution of 1:10 is recommended, based on the Indirect method, with a normal ABC method the dilutions can be 5-10x higher, but the optimal dilution should be tested by serial dilution.

0.1% Sodium Azide

Store undiluted at 2-8°C for 2 months. For long term storage, aliquot and store at -20°C. Avoid freeze-thaw cycles.

For research use only. Intended for use by laboratory professionals.

This antibody carries the LSBio 100% Guarantee.

Uniprot: P08247 NCBI: X06389 CAA29686.1