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Catalog Number Size Price
LS-C799226-100 100 µg $539 
SYP / Synaptophysin Antibody - Immunohistochemical staining of paraffin-embedded human brain using anti-SYP clone UMAB112 mouse monoclonal antibody at 1:800 dilution 1mg/mL and detection with Polink2 Broad HRP DAB.requires heat-induced epitope retrieval with citrate pH6.0 in a presure cooker for 3 minutes at 110C. The image shows strong membranous and cytoplasmic staining in granular layer and purkinje cells.
SYP / Synaptophysin Antibody - Immunohistochemical staining of paraffin-embedded Human adult brain tissue using anti-SYP mouse monoclonal antibody.  heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min)
SYP / Synaptophysin Antibody - Immunohistochemical staining of paraffin-embedded human pancreas using anti-SYP clone UMAB112 mouse monoclonal antibody at 1:200 dilution of 0.4mg/mL detection with Polink2 Broad HRP DAB.requires heat-induced epitope retrieval with Citrate pH6.0 at 95-100C 20 minutes. The image shows strong membranous and cytoplasmic staining in the Islets of Langerhans with no staining in the glandular cells.
SYP / Synaptophysin Antibody - Immunohistochemical staining of paraffin-embedded human carcinoid tumor using anti-SYP clone UMAB112 mouse monoclonal antibody at 1:200 dilution of 0.4mg/mL and detection with Polink2 Broad HRP DAB.requires heat-induced epitope retrieval with Citrate pH6.0 at 95-100C 20 minutes. The image shows strong membranous and cytoplasmic staining in the tumor cells with surrounding stroma negative.
SYP / Synaptophysin Antibody - Confocal immunofluoresce image of primary rat neurons labeled with anti-SYP mouse monoclonal antibody  green, 1:100). Actin filaments were labeled with TRICT-Phalloidin. (red), and nuclear with DAPI. (blue). Scale bar, 20um.
SYP / Synaptophysin Antibody - Immunofluorescent staining of SH-SY5Y cells using anti-SYP mouse monoclonal antibody  green, 1:100). Actin filaments were labeled with Alexa Fluor® 594 Phalloidin. (red), and nuclear with DAPI. (blue). Scale bar, 20µm.
SYP / Synaptophysin Antibody - Western blot of mouse tissue lysates. (20ug) from Brain. Primary antibody diluation: 1:500. Secondary antibody dilution: Mouse TrueBlot® Ultra. (1:1000).
SYP / Synaptophysin Antibody - Western blot of cell lysates. (35ug) from 9 different cell lines. (1: HepG2, 2: HeLa, 3: SV-T2, 4: A549. 5: COS7, 6: Jurkat, 7: PC-12, 8: MDCK, 9: MCF7).
SYP / Synaptophysin Antibody - Western blot of human tissue lysates. (15ug) from 10 different tissues. (1: Testis, 2: Omentum, 3: Uterus, 4: Breast, 5: Brain, 6: Liver, 7: Ovary, 8: Thyroid, 9: Colon, 10: Spleen). Diluation: 1:500.
SYP / Synaptophysin Antibody - OriGene overexpression protein microarray chip was immunostained with UltraMAB anti-SYP mouse monoclonal antibody. The positive reactive proteins are highlighted with two red arrows in the enlarged subarray. All the positive controls spotted in this subarray are also labeled for clarification.
SYP / Synaptophysin Antibody - Immunohistochemical staining of paraffin-embedded human brain using anti-SYP clone UMAB112 mouse monoclonal antibody at 1:800 dilution 1mg/mL and detection with Polink2 Broad HRP DAB.requires heat-induced epitope retrieval with citrate pH6.0 in a presure cooker for 3 minutes at 110C. The image shows strong membranous and cytoplasmic staining in granular layer and purkinje cells.
SYP / Synaptophysin Antibody - Immunohistochemical staining of paraffin-embedded Human adult brain tissue using anti-SYP mouse monoclonal antibody.  heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min)
SYP / Synaptophysin Antibody - Immunohistochemical staining of paraffin-embedded human pancreas using anti-SYP clone UMAB112 mouse monoclonal antibody at 1:200 dilution of 0.4mg/mL detection with Polink2 Broad HRP DAB.requires heat-induced epitope retrieval with Citrate pH6.0 at 95-100C 20 minutes. The image shows strong membranous and cytoplasmic staining in the Islets of Langerhans with no staining in the glandular cells.
SYP / Synaptophysin Antibody - Immunohistochemical staining of paraffin-embedded human carcinoid tumor using anti-SYP clone UMAB112 mouse monoclonal antibody at 1:200 dilution of 0.4mg/mL and detection with Polink2 Broad HRP DAB.requires heat-induced epitope retrieval with Citrate pH6.0 at 95-100C 20 minutes. The image shows strong membranous and cytoplasmic staining in the tumor cells with surrounding stroma negative.
SYP / Synaptophysin Antibody - Confocal immunofluoresce image of primary rat neurons labeled with anti-SYP mouse monoclonal antibody  green, 1:100). Actin filaments were labeled with TRICT-Phalloidin. (red), and nuclear with DAPI. (blue). Scale bar, 20um.
SYP / Synaptophysin Antibody - Immunofluorescent staining of SH-SY5Y cells using anti-SYP mouse monoclonal antibody  green, 1:100). Actin filaments were labeled with Alexa Fluor® 594 Phalloidin. (red), and nuclear with DAPI. (blue). Scale bar, 20µm.
SYP / Synaptophysin Antibody - Western blot of mouse tissue lysates. (20ug) from Brain. Primary antibody diluation: 1:500. Secondary antibody dilution: Mouse TrueBlot® Ultra. (1:1000).
SYP / Synaptophysin Antibody - Western blot of cell lysates. (35ug) from 9 different cell lines. (1: HepG2, 2: HeLa, 3: SV-T2, 4: A549. 5: COS7, 6: Jurkat, 7: PC-12, 8: MDCK, 9: MCF7).
SYP / Synaptophysin Antibody - Western blot of human tissue lysates. (15ug) from 10 different tissues. (1: Testis, 2: Omentum, 3: Uterus, 4: Breast, 5: Brain, 6: Liver, 7: Ovary, 8: Thyroid, 9: Colon, 10: Spleen). Diluation: 1:500.
SYP / Synaptophysin Antibody - OriGene overexpression protein microarray chip was immunostained with UltraMAB anti-SYP mouse monoclonal antibody. The positive reactive proteins are highlighted with two red arrows in the enlarged subarray. All the positive controls spotted in this subarray are also labeled for clarification.
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Monoclonal Mouse anti‑Human SYP / Synaptophysin Antibody (clone UMAB112, Carrier‑free, IHC, IF, WB) LS‑C799226

Monoclonal Mouse anti‑Human SYP / Synaptophysin Antibody (clone UMAB112, Carrier‑free, IHC, IF, WB) LS‑C799226

Antibody:
SYP / Synaptophysin Mouse anti-Human Monoclonal (Carrier-free) (UMAB112) Antibody
Application:
IHC, IF, WB, PMA
Reactivity:
Human
Format:
Unconjugated, Carrier-free
Other formats:
Price
Catalog Number
$539
LS-C799226-100
Toll Free North America
(800) 227-6666
For Research Use Only

Overview

Antibody:
SYP / Synaptophysin Mouse anti-Human Monoclonal (Carrier-free) (UMAB112) Antibody
Application:
IHC, IF, WB, PMA
Reactivity:
Human
Format:
Unconjugated, Carrier-free
Other formats:

Specifications

Description
Synaptophysin antibody LS-C799226 is an unconjugated mouse monoclonal antibody to human Synaptophysin (SYP). Validated for IF, IHC, PMA and WB.
Target
Human SYP / Synaptophysin
Synonyms
SYP | MRX | Synaptophysin | MRXSYP
Host
Mouse
Reactivity
Human (tested or 100% immunogen sequence identity)
Clonality
IgG2b Monoclonal
Clone
UMAB112
Conjugations
Unconjugated
Purification
Affinity chromatography
Modifications
Carrier-free. Also available Unmodified.
Immunogen
Full lengthu human recombinant protein of human SYP(NP_003170) produced in HEK293T cell.
Specificity
The specificity of this antibody has been validated by testing against a high-density Protein Microarray containing more than 17,000 recombinant proteins.
Applications
  • IHC (1:100 - 1:200)
  • Immunofluorescence
  • Western blot
  • Protein Microarray
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Usage
Applications should be user optimized.
Presentation
PBS, pH 7.3, 8% Trehalose
Reconstitution
Reconstitute with PBS pH 7.3. To use this carrier-free antibody for conjugation experiment, we strongly recommend you to perform another round of desalting process.
Storage
Store at -20°C. Avoid freeze-thaw cycles.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee
About SYP / Synaptophysin
P08247 X06389 CAA29686.1

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Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at:

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Requested From: United States
Date Requested: 11/23/2024