Human TNF Alpha

Mouse

Human (tested or 100% immunogen sequence identity)

IgG1 Monoclonal

Unconjugated

Protein G purified

Unmodified

RhTNR-a expressed in E. coli.

Recognizes recombinant human TNF-a. Cross-reactivity: rhTNF-b, rmTNF-a, rrTNF-a, rpTNF-a, rhTNF RI, rhTNF RII, rmTNF RI and rmTNF RII: >0.05%.

• ICC
• Western blot (1 - 2 µg/ml)
• ELISA (4 µg/ml)
• Neutralization

Suitable for use in ELISA, Western Blot, Immunocytochemistry and Neutralization. ELISA: 4 ug/ml as a capture antibody. Use 100 ul/well. Western Blot: ~1-2 ug/ml. The detection limit for rhTNFa is 5 ng/lane under non-reducing and reducing conditions using colorimetric detection. 5-50-fold increased sensitivity can be obtained using ECL. Neutralization of Bioactivity: 0.04-0.08 ug/ml typically neutralizes 50% of the bioactivity of 0.25 ng/ml of rhTNF-a.

PBS, pH 7.2, 5% Trehalose

Short term: store at 4°C. Long term: aliquot and store at -20°C. Avoid freeze-thaw cycles.

For research use only. Intended for use by laboratory professionals.

This antibody carries the LSBio 100% Guarantee.

Uniprot: P01375 NCBI: NM_000594 NP_000585.2