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Catalog Number Size Price
LS-C352952-100 100 µl (1 mg/ml) $299 
TNFRSF1B / TNFR2 Antibody - Immunohistochemical analysis of CD120b staining in human lung cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with hematoxylin and mounted with DPX.
TNFRSF1B / TNFR2 Antibody - Immunofluorescent analysis of CD120b staining in PC12 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue).
TNFRSF1B / TNFR2 Antibody - Western blot analysis of CD120b expression in HEK293T (A); mouse heart (B); rat heart (C); PC12 (D) whole cell lysates.
TNFRSF1B / TNFR2 Antibody - Immunohistochemical analysis of CD120b staining in human lung cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with hematoxylin and mounted with DPX.
TNFRSF1B / TNFR2 Antibody - Immunofluorescent analysis of CD120b staining in PC12 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue).
TNFRSF1B / TNFR2 Antibody - Western blot analysis of CD120b expression in HEK293T (A); mouse heart (B); rat heart (C); PC12 (D) whole cell lysates.
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Polyclonal Rabbit anti‑Human TNFRSF1B / TNFR2 Antibody (C‑Terminus, IHC, IF, WB) LS‑C352952

Polyclonal Rabbit anti‑Human TNFRSF1B / TNFR2 Antibody (C‑Terminus, IHC, IF, WB) LS‑C352952

Antibody:
TNFRSF1B / TNFR2 Rabbit anti-Human Polyclonal (C-Terminus) Antibody
Application:
IHC, IHC-P, ICC, IF, WB, IP
Reactivity:
Human, Mouse, Rat
Format:
Unconjugated, Unmodified
Price
Catalog Number
$299
LS-C352952-100
Toll Free North America
(800) 227-6666
For Research Use Only

Overview

Antibody:
TNFRSF1B / TNFR2 Rabbit anti-Human Polyclonal (C-Terminus) Antibody
Application:
IHC, IHC-P, ICC, IF, WB, IP
Reactivity:
Human, Mouse, Rat
Format:
Unconjugated, Unmodified

Specifications

Description
TNFR2 antibody LS-C352952 is an unconjugated rabbit polyclonal antibody to TNFR2 (TNFRSF1B) (C-Terminus) from human. It is reactive with human, mouse and rat. Validated for ICC, IF, IHC, IP and WB.
Target
Human TNFRSF1B / TNFR2
Synonyms
TNFRSF1B | CD120b antigen | CD120b | p75 TNF receptor | Soluble TNFR1B variant 1 | TNFBR | Tumor necrosis factor receptor | TNF-R-II | TNF-R75 | TNFR2 | p75TNFR | p80 TNF-alpha receptor | TBPII | TNF-R2 | TNFR80 | TNF-RII | TNFR-II | TNFR1B
Host
Rabbit
Reactivity
Human, Mouse, Rat (tested or 100% immunogen sequence identity)
Clonality
Polyclonal
Conjugations
Unconjugated
Purification
Immunoaffinity purified
Modifications
Unmodified
Immunogen
KLH-conjugated synthetic peptide encompassing a sequence within the C-Terminus of human CD120b.
Epitope
C-Terminus
Specificity
Recognizes endogenous levels of CD120b protein.
Applications
  • IHC
  • IHC - Paraffin (1:100 - 1:200)
  • ICC (1:100 - 1:500)
  • Immunofluorescence
  • Western blot (1:500 - 1:1000)
  • Immunoprecipitation (1:10 - 1:100)
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Presentation
PBS, pH 7.3, 0.01% Sodium Azide, 30% Glycerol
Storage
Store at -20°C. Aliquot to avoid freeze/thaw cycles.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee
About TNFRSF1B / TNFR2
P20333 NM_001066 NP_001057.1

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Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at:

Technical.Support@LSBio.com

Requested From: United States
Date Requested: 11/23/2024