Mouse TNFRSF1B / TNFR2

Rabbit

Mouse (tested or 100% immunogen sequence identity)

IgG Monoclonal

R204

Unconjugated

Protein A purified

Preservative Free

Recombinant Mouse TNFR2/TNFRSF1B/CD120b protein

Mouse TNFR2/TNFRSF1B. No cross-reactivity with Human TNFR2/TNFRSF1B, or Cynomolgus TNFR2/TNFRSF1B in ELISA assay

• ELISA (0.4 µg/ml)
• Blocking (0.5 µg/ml)
• Neutralization (25 ng/ml)

Block: In a functional ELISA which immobilized Mouse TNFa at 0.4 ug/ml (100 µL/well) in the plate, the Rabbit anti-Mouse TNFR2/TNFRSF1B Monoclonal Antibody can block the binding of 0.5 ug/ml of biotinylated Mouse TNFR2/TNFRSF1B to Mouse TNFa, the EC50 is 0.2-0.7 ug/ml. Neutralization: The neutralization activity of mouse TNFRSF1B neutralizing antibody is measured by its ability to neutralize mTNFRSF1B-mediated inhibition of cytotoxicity in the L-929 mouse fibroblast cell line. The Neutralization Dose (ND50) is typically 16-50 ng/mL in the presence of 25ng/mL mTNFRSF1B, 0.5ng/mL mouse TNFa, and 1ug/mL actinomycin D. Please Note: Optimal concentrations/dilutions should be determined by the end user.

0.2 µm filtered histidine and arginine buffer containing 120mM NaCl, 0.02% Tween 80, pH 6.0

Store at 2°C to 8°C for up to 1 month. Aliquot and store at -20°C to -80°C for up to 1 year. Avoid freeze/thaw cycles.

For research use only. Intended for use by laboratory professionals.

This antibody carries the LSBio 100% Guarantee.

Uniprot: P20333 NCBI: NM_001066 NP_001057.1