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Antibodies > TNFSF11 / RANKL / TRANCE > LS-C358364
 
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Catalog Number Size Price
LS-C358364-100 100 µl (1 mg/ml) $299 
TNFSF11 / RANKL / TRANCE Antibody - Immunohistochemical analysis of CD254 staining in human lymph node formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP-conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with hematoxylin and mounted with DPX.
TNFSF11 / RANKL / TRANCE Antibody - Immunofluorescent analysis of CD254 staining in Jurkat cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 deg C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue).
TNFSF11 / RANKL / TRANCE Antibody - Western blot analysis of CD254 expression in Jurkat (A); Raji (B) whole cell lysates.
TNFSF11 / RANKL / TRANCE Antibody - Immunohistochemical analysis of CD254 staining in human lymph node formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP-conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with hematoxylin and mounted with DPX.
TNFSF11 / RANKL / TRANCE Antibody - Immunofluorescent analysis of CD254 staining in Jurkat cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 deg C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue).
TNFSF11 / RANKL / TRANCE Antibody - Western blot analysis of CD254 expression in Jurkat (A); Raji (B) whole cell lysates.
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Polyclonal Rabbit anti‑Human TNFSF11 / RANKL / TRANCE Antibody (Internal, IHC, IF, WB) LS‑C358364

Polyclonal Rabbit anti‑Human TNFSF11 / RANKL / TRANCE Antibody (Internal, IHC, IF, WB) LS‑C358364

Submit Review LSBio Guarantee
Antibody:
TNFSF11 / RANKL / TRANCE Rabbit anti-Human Polyclonal (Internal) Antibody
Application:
IHC, IHC-P, ICC, IF, WB
Reactivity:
Human
Format:
Unconjugated, Unmodified
Price
Catalog Number
$299
LS-C358364-100
Toll Free North America
(800) 227-6666
For Research Use Only
Specifications Validation Publications Reviews Featured Products Request SDS/MSDS

Overview

Antibody:
TNFSF11 / RANKL / TRANCE Rabbit anti-Human Polyclonal (Internal) Antibody
Application:
IHC, IHC-P, ICC, IF, WB
Reactivity:
Human
Format:
Unconjugated, Unmodified

Specifications

Description
TRANCE antibody LS-C358364 is an unconjugated rabbit polyclonal antibody to human TRANCE (TNFSF11 / RANKL) (Internal). Validated for ICC, IF, IHC and WB.
Target
Human TNFSF11 / RANKL / TRANCE
Synonyms
TNFSF11 | CD254 | CD254 antigen | HRANKL2 | OPTB2 | TRANCE | ODF | OPGL | SOdf | Osteoprotegerin ligand | RANKL
Host
Rabbit
Reactivity
Human (tested or 100% immunogen sequence identity)
Clonality
Polyclonal
Conjugations
Unconjugated
Purification
Immunoaffinity purified
Modifications
Unmodified
Immunogen
KLH-conjugated synthetic peptide encompassing a sequence within the central region of human CD254.
Epitope
Internal
Specificity
Recognizes endogenous levels of CD254 protein.
Applications
  • IHC
  • IHC - Paraffin (1:100 - 1:200)
  • ICC (1:100 - 1:500)
  • Immunofluorescence
  • Western blot (1:500 - 1:1000)
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Presentation
PBS, pH 7.3, 0.01% Sodium Azide, 30% Glycerol
Storage
Store at -20°C. Aliquot to avoid freeze/thaw cycles.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee
About TNFSF11 / RANKL / TRANCE
O14788 AF053712 AAC39731.1

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Featured Products

TNFSF11 / RANKL / TRANCE Antibody - Western blot of TRANCE/RANKL in transfected cells. A single protein band of approximate mol. wt. of 35 kD was detected. This image was taken for the unmodified form of this product. Other forms have not been tested.
TNFSF11 / RANKL / TRANCE Antibody (aa1‑317, Azide‑free) LS‑C19335
Species: Mouse, Human
Applications: IHC, IHC - Paraffin, Western blot
TNFSF11 / RANKL / TRANCE Antibody - Anti-RANKL antibody IHC of human small intestine. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.
IHC‑plus™ TNFSF11 / RANKL / TRANCE Antibody LS‑B1425
Species: Human, Mouse, Rat
Applications: IHC, IHC - Paraffin, Western blot, ELISA
TNFSF11 / RANKL / TRANCE Antibody - Biotinylated Anti-Human sRANK Ligand (Polyclonal Rabbit) Western Blot Reduced
TNFSF11 / RANKL / TRANCE Antibody (Biotin) LS‑C104393
Species: Human
Applications: Western blot, ELISA
Product
TNFSF11 / RANKL / TRANCE Antibody (aa64‑245, clone TS106) LS‑C191486
Species: Human
Applications: Western blot
TNFSF11 / RANKL / TRANCE Antibody - Western Blot (reducing) of RANKL antibody. This image was taken for the unconjugated form of this product. Other forms have not been tested.
TNFSF11 / RANKL / TRANCE Antibody LS‑C104767
Species: Mouse
Applications: Western blot, ELISA
TNFSF11 / RANKL / TRANCE Antibody - Sandwich ELISA of RANKL antibody
TNFSF11 / RANKL / TRANCE Antibody LS‑C104835
Species: Human
Applications: ELISA
See all TNFSF11 / RANKL / TRANCE Antibodies

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at:

Technical.Support@LSBio.com
Requested From: United States
Date Requested: 12/22/2024
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For technical assistance:
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