Human TP53 / p53 Mutant

Mouse

Human (tested or 100% immunogen sequence identity)

IgG2a Monoclonal

TRP/816

Unconjugated

Protein G affinity chromatography

Unmodified. Also available Azide-free, BSA-free, Ready-to-Use.

A recombinant protein was used as immunogen.

This antibody has been extensively validated, including cross-reactivity testing against 19,000 full-length proteins by HuProt(TM) microarray analysis.

• IHC - Paraffin (0.5 - 1 µg/ml)
• Immunofluorescence (1 - 2 µg/ml)
• Western blot (0.5 - 1 µg/ml)
• Flow Cytometry (0.5 - 1 µg/10E6 cells)

Optimal dilution of the TP53 antibody should be determined by the researcher. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min. Prediluted IHC only format: incubate for 30 min at RT

1X PBS, 0.1 mg/ml BSA, 0.05% sodium azide

Store at 2-8°C (with azide) or aliquot and store at -20°C (without azide).

For research use only. Intended for use by laboratory professionals.

This antibody carries the LSBio 100% Guarantee.