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Catalog Number Size Price
LS-C104581-100 100 µg $396 
VEGFA / VEGF Antibody - Western Blot (reducing) of VEGF antibody
VEGFA / VEGF Antibody - Western Blot (non-reducing) of VEGF antibody
VEGFA / VEGF Antibody - Sandwich ELISA of VEGF antibody
VEGFA / VEGF Antibody - Western Blot (reducing) of VEGF antibody
VEGFA / VEGF Antibody - Western Blot (non-reducing) of VEGF antibody
VEGFA / VEGF Antibody - Sandwich ELISA of VEGF antibody
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Polyclonal Rabbit anti‑Human VEGFA / VEGF Antibody (WB) LS‑C104581

Polyclonal Rabbit anti‑Human VEGFA / VEGF Antibody (WB) LS‑C104581

Antibody:
VEGFA / VEGF Rabbit anti-Human Polyclonal Antibody
Application:
WB, ELISA
Reactivity:
Human
Format:
Unconjugated, Unmodified
Other formats:
Price
Catalog Number
$396
LS-C104581-100
Toll Free North America
206-374-1102
For Research Use Only

Overview

Antibody:
VEGFA / VEGF Rabbit anti-Human Polyclonal Antibody
Application:
WB, ELISA
Reactivity:
Human
Format:
Unconjugated, Unmodified
Other formats:

Specifications

Description
VEGF antibody LS-C104581 is an unconjugated rabbit polyclonal antibody to human VEGF (VEGFA). Validated for ELISA and WB.
Target
Human VEGFA / VEGF
Synonyms
VEGFA | VPF | Vascular permeability factor | VEGF | VEGF-A | MVCD1
Host
Rabbit
Reactivity
Human (tested or 100% immunogen sequence identity)
Clonality
Polyclonal
Conjugations
Unconjugated. Also available conjugated with Biotin.
Purification
Immunoaffinity purified
Modifications
Unmodified
Immunogen
E.coli derived recombinant Human VEGF165.
Specificity
Human VEGF
Applications
  • Western blot (0.1 - 0.2 µg/ml)
  • ELISA (0.5 - 2 µg/ml)
Usage
Immunohistochemistry: This antibody stained formalin-fixed, paraffin-embedded sections of Human breast invasive ductal carcinoma. The recommended concentrations are 2.5-5.5 mg/ml with an overnight incubation at 4°C. An HRP-labeled polymer detection system was used with a DAB chromogen. Heat induced antigen retrieval with a pH 6 Sodium Citrate buffer is recommended. Optimal concentrations and conditions may vary. Neutralization: To yield one-half maximal inhibition [ND] of the biological activity of hVEGF (10 ng/ml), a concentration of 0.05-0.1 ug/ml of this antibody is required. ELISA: To detect hVEGF by sandwich ELISA (using 100 ul/well antibody solution) a concentration of 0.5-2 ug/ml of this antibody is required. This antigen affinity purified antibody, in conjunction with Biotinylated Anti-Human VEGF (LS-C104615) as a detection antibody, allows the detection of at least 0.2-0.4 ng/well of recombinant hVEGF. Western Blot: To detect hVEGF by Western Blot analysis this antibody can be used at a concentration of 0.1-0.2 ug/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant hVEGF is 1.5-3 ng/lane, under either reducing or non-reducing conditions.
Presentation
Lyophilized from PBS, pH 7.2
Reconstitution
Centrifuge vial prior to opening. Reconstitiute in sterile water to 0.1-1.0 mg/ml. Do not vortex.
Storage
Store Lyophilized at room temperature up to 1 month; Reconstituted for up to 2 weeks at 2°C to 8°C. Aliquot and freeze at -20°C for long term storage. Avoid freeze/thaw cycles.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee
About VEGFA / VEGF
P15692 NM_003376 NP_003367.4

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To request an SDS/MSDS form for this product, please contact our Technical Support department at:

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Requested From: United States
Date Requested: 10/4/2024