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Catalog Number Size Price
LS-K244-100 100 Tests $550 
Acetylcholine Assay Kit

Acetylcholine Assay Kit (Colorimetric/Fluorometric) - LS-K244

Acetylcholine Assay Kit (Colorimetric/Fluorometric) - LS-K244

Available for shipment within the USA only
Description:
ACETYLCHOLINE is a neurotransmitter produced in acetylcholinergic neurons. It plays important roles in skeletal muscle movement, regulation of smooth and cardiac muscles, as well as in learning, memory and mood. This Method provides a simple, direct and high-throughput assay for measuring acetylcholine in biological samples. In this assay, acetylcholine is hydrolyzed by acetylcholinesterase to choline which is oxidized by choline oxidase to betaine and H2O2. The resulting H2O2 reacts with a specific dye to form a pink colored product. The color intensity at 570nm or fluorescence intensity (530/585 nm) is directly proportional to the acetylcholine concentration in the sample.
Price
Catalog Number
$550
LS-K244-100

Available for USA Shipment Only
Toll Free North America
(800) 227-6666
For Research Use Only

Overview

Description:
ACETYLCHOLINE is a neurotransmitter produced in acetylcholinergic neurons. It plays important roles in skeletal muscle movement, regulation of smooth and cardiac muscles, as well as in learning, memory and mood. This Method provides a simple, direct and high-throughput assay for measuring acetylcholine in biological samples. In this assay, acetylcholine is hydrolyzed by acetylcholinesterase to choline which is oxidized by choline oxidase to betaine and H2O2. The resulting H2O2 reacts with a specific dye to form a pink colored product. The color intensity at 570nm or fluorescence intensity (530/585 nm) is directly proportional to the acetylcholine concentration in the sample.

Specifications

Name
Acetylcholine Assay Kit (Colorimetric/Fluorometric)
Type
Detection/Quantition
Usage
For quantitative determination of acetylcholine and evaluation of drug effects on acetylcholine metabolism.
Target
Acetylcholine
SampleType
Cell Cultures, Plasma, Serum, Saliva, Tissue, Urine
Detection
Colorimetric (570 nm Absorbance), Fluorometric (Ex/Em 530/585 nm)
Supplied Components
The following components are supplied with this product.
  • Assay Buffer
  • ACHE Enzyme
  • Enzyme Mix: Dried
  • Standard
  • Dye Reagent
  • (See Datasheet for specific volumes supplied)
Applications
Spectrophotometry (fluorometric), Spectrophotometry (visible)
Equipment
Microplate fluorometer, Microplate spectrophotometer (visible)
Conditions
Shipped -20°C Dry Ice, Store at -20°C, 3 months shelf life.
Documents
Restrictions
For research use only. Intended for use by laboratory professionals. Available for shipment within the USA only
Guarantee
This Assay Kit carries the LSBio 100% Guarantee

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Colorimetry

Acetylcholine Assay Kit

Colorimetry

Acetylcholine Assay Kit

Colorimetry

Acetylcholine Assay Kit

Colorimetry

Acetylcholine Assay Kit

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at:

Technical.Support@LSBio.com

Requested From: United States
Date Requested: 11/24/2024