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Catalog Number Size Price
LS-K306-100 100 Tests $435 
Lactate Dehydrogenase Assay Kit

Lactate Dehydrogenase (LDH) Assay Kit (Colorimetric) - LS-K306

Lactate Dehydrogenase (LDH) Assay Kit (Colorimetric) - LS-K306

Available for shipment within the USA only
Description:
LACTATE DEHYDROGENASE (LDH) is an oxidoreductase which catalyzes the interconversion of lactate and pyruvate. When disease or injury affects tissues containing LDH, the cells release LDH into the bloodstream, where it is identified in higher than normal levels. Therefore, LDH is most often measured to evaluate the presence of tissue or cell damage. The non-radioactive colorimetric LDH assay is based on the reduction of the tetrazolium salt MTT in a NADH-coupled enzymatic reaction to a reduced form of MTT which exhibits an absorption maximum at 565 nm. The intensity of the purple color formed is directly proportional to the enzyme activity.
Price
Catalog Number
$435
LS-K306-100

Available for USA Shipment Only
Toll Free North America
206-374-1102
For Research Use Only

Overview

Description:
LACTATE DEHYDROGENASE (LDH) is an oxidoreductase which catalyzes the interconversion of lactate and pyruvate. When disease or injury affects tissues containing LDH, the cells release LDH into the bloodstream, where it is identified in higher than normal levels. Therefore, LDH is most often measured to evaluate the presence of tissue or cell damage. The non-radioactive colorimetric LDH assay is based on the reduction of the tetrazolium salt MTT in a NADH-coupled enzymatic reaction to a reduced form of MTT which exhibits an absorption maximum at 565 nm. The intensity of the purple color formed is directly proportional to the enzyme activity.

Specifications

Name
Lactate Dehydrogenase (LDH) Assay Kit (Colorimetric)
Type
Detection/Quantition
Usage
For quantitative determination of lactate dehydrogenase LDH activity and screen/evaluation of LDH modulators.
Target
Lactate Dehydrogenase
SampleType
Plasma, Serum
Detection
Colorimetric (565 nm Absorbance)
Supplied Components
The following components are supplied with this product.
  • Substrate Buffer
  • Diaphorase
  • NAD Solution
  • Calibrator
  • MTT Solution
  • (See Datasheet for specific volumes supplied)
Applications
Spectrophotometry (visible)
Equipment
Microplate spectrophotometer (visible), Spectrophotometer (visible)
Conditions
Shipped Ambient, Store at 4°C or -20°C, 6 months shelf life.
Documents
Restrictions
For research use only. Intended for use by laboratory professionals. Available for shipment within the USA only
Guarantee
This Assay Kit carries the LSBio 100% Guarantee

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Colorimetry

Lactate Dehydrogenase Assay Kit

Colorimetry

Lactate Dehydrogenase Assay Kit

Colorimetry

Lactate Dehydrogenase Assay Kit

Colorimetry

Lactate Dehydrogenase Assay Kit

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at:

Technical.Support@LSBio.com

Requested From: United States
Date Requested: 6/16/2024