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Catalog Number Size Price
LS-K241-5000 5000 Tests $280 
LS-K241-10000 10000 Tests $420 
Cell Viability Assay Kit

Resorufin Blue Cell Viability Assay Kit (Fluorometric) - LS-K241

Resorufin Blue Cell Viability Assay Kit (Fluorometric) - LS-K241

Available for shipment within the USA only
Description:
This homogeneous assay involves simply adding a single reagent, the reagent, to the cell culture and measuring the fluorescence intensity (excitation wavelength = 530 - 570 nm, emission wavelength = 590 - 620 nm) after an incubation step. The reagent, like other resazurin-based assays such as the Alamar Blue reagent, utilizes the redox dye resazurin which is not fluorescent, but upon reduction by metabolically active cells is converted into a highly fluorescent product (resorufin). Living cells can readily reduce this non-toxic reagent and the resulting increase in fluorescence intensity can be conveniently monitored using a fluorescence spectrophotometer or plate reader. Nonviable cells have no metabolic capacity and, thus, will not reduce the dye. Therefore, the fluorescence intensity observed in this assay is a true measure of the viable cells. The reagent has been optimized for maximum sensitivity, reproducibility and long shelf-life. The homogeneous cell-based assay can be performed in multi-well plates. The reagent is compatible with all culture media and with all liquid handling systems for high-throughput screening applications in 96-well and 384-well plates. Applications include cell proliferation, cytotoxicity and apoptosis.
Price
Catalog Number
$280
LS-K241-5000

Available for USA Shipment Only
Toll Free North America
206-374-1102
For Research Use Only

Overview

Description:
This homogeneous assay involves simply adding a single reagent, the reagent, to the cell culture and measuring the fluorescence intensity (excitation wavelength = 530 - 570 nm, emission wavelength = 590 - 620 nm) after an incubation step. The reagent, like other resazurin-based assays such as the Alamar Blue reagent, utilizes the redox dye resazurin which is not fluorescent, but upon reduction by metabolically active cells is converted into a highly fluorescent product (resorufin). Living cells can readily reduce this non-toxic reagent and the resulting increase in fluorescence intensity can be conveniently monitored using a fluorescence spectrophotometer or plate reader. Nonviable cells have no metabolic capacity and, thus, will not reduce the dye. Therefore, the fluorescence intensity observed in this assay is a true measure of the viable cells. The reagent has been optimized for maximum sensitivity, reproducibility and long shelf-life. The homogeneous cell-based assay can be performed in multi-well plates. The reagent is compatible with all culture media and with all liquid handling systems for high-throughput screening applications in 96-well and 384-well plates. Applications include cell proliferation, cytotoxicity and apoptosis.

Specifications

Name
Resorufin Blue Cell Viability Assay Kit (Fluorometric)
Type
Cell Viability
Usage
Homogeneous assay for cell viability, proliferation, cytotoxcity and high-throughput screen for anticancer agents. As low as 100 cells can be accurately quantified.
Target
Cell Viability
SampleType
Cell Cultures
Detection
Fluorometric (Ex/Em 530/590 nm)
Supplied Components
The following components are supplied with this product.
  • Reagent
  • Control Reagent
  • (See Datasheet for specific volumes supplied)
Applications
Spectrophotometry (fluorometric)
Equipment
Microplate fluorometer
Conditions
Shipped Ambient, Store at 4°C, 12 months shelf life.
Documents
Restrictions
For research use only. Intended for use by laboratory professionals. Available for shipment within the USA only
Guarantee
This Assay Kit carries the LSBio 100% Guarantee

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Fluorimetry

Cell Viability Assay Kit

Fluorimetry

Cell Viability Assay Kit

Fluorimetry

Cell Viability Assay Kit

Fluorimetry

Cell Viability Assay Kit

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at:

Technical.Support@LSBio.com

Requested From: United States
Date Requested: 6/16/2024