Human ANO1 / DOG1 / TMEM16A

Rabbit

Human (tested or 100% immunogen sequence identity)

IgG Recombinant Monoclonal

DG1/2564R

Unconjugated

Protein A/G purified

Unmodified. Also available Azide-free, Carrier-free.

Recombinant human DOG-1 protein fragment (aa 2-101). Antigen Molecular Weight: ~114 kDa

aa 2-101

The expression of DOG1 is elevated in the gastrointestinal stromal tumors (GISTs), c-kit signaling-driven mesenchymal tumors of the GI tract. DOG1 is rarely expressed in other soft tissue tumors, which, due to appearance, may be difficult to diagnose. Immunoreactivity for DOG1 has been reported in 97.8 percent of scorable GIST s, including all c-kit negative GISTs. Overexpression of DOG1 has been suggested to aid in the identification of GISTs, including Platelet-Derived Growth Factor Receptor Alpha mutants that fail to express c-kit antigen. The overall sensitivity of DOG1 and c-kit in GIST s is nearly identical: 94.4% vs. 94.7%.

• IHC - Fixed (1:50)

Immunohistology (Formalin-fixed - 0.5-1.0 ug/ml for 30 minutes at RT - Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes.) Optimal dilution for a specific application should be determined.

10 mM PBS, 0.05% BSA, 0.05% Sodium Azide

Store at 2°C to 8°C for up to 2 years.

For research use only. Intended for use by laboratory professionals.

This antibody carries the LSBio 100% Guarantee.

Uniprot: Q5XXA6 NCBI: NM_018043 NP_060513.5