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Catalog Number Size Price
LS-B3337-250 250 µl (0.2 mg/ml) $440 
CCNE1 / Cyclin E1 Antibody - Human Testis: Formalin-Fixed, Paraffin-Embedded (FFPE)
CCNE1 / Cyclin E1 Antibody - Human Placenta: Formalin-Fixed, Paraffin-Embedded (FFPE)
CCNE1 / Cyclin E1 Antibody - Human Testis: Formalin-Fixed, Paraffin-Embedded (FFPE)
CCNE1 / Cyclin E1 Antibody - Human Placenta: Formalin-Fixed, Paraffin-Embedded (FFPE)
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PathPlus™ Polyclonal Rabbit anti‑Human CCNE1 / Cyclin E1 Antibody (C‑Terminus, IHC) LS‑B3337

PathPlus™ Polyclonal Rabbit anti‑Human CCNE1 / Cyclin E1 Antibody (C‑Terminus, IHC) LS‑B3337

Note: This antibody replaces LS-C50626
Antibody:
CCNE1 / Cyclin E1 Rabbit anti-Human Polyclonal (C-Terminus) Antibody
Application:
IHC-P
Reactivity:
Human, Mouse
Format:
Unconjugated, Unmodified
Price
Catalog Number
$440
LS-B3337-250
Toll Free North America
(800) 227-6666
For Research Use Only

Overview

Antibody:
CCNE1 / Cyclin E1 Rabbit anti-Human Polyclonal (C-Terminus) Antibody
Application:
IHC-P
Reactivity:
Human, Mouse
Format:
Unconjugated, Unmodified

Specifications

Description
CCNE1 (Cyclin E1) belongs to the highly conserved cyclin family, whose members are characterized by a dramatic periodicity in protein abundance through the cell cycle. It forms a complex with and functions as a regulatory subunit of CDK2, whose activity is required for cell cycle G1/S transition. This protein accumulates at the G1-S phase boundary and is degraded as cells progress through S phase. Overexpression of this gene has been observed in many tumors, which results in chromosome instability, and thus may contribute to tumorigenesis. This protein is involved in the phosphorylation of NPAT protein, which participates in cell-cycle regulated histone gene expression and plays a critical role in promoting cell-cycle progression in the absence of pRB. In immunohistochemistry, CCNE1 has variable levels of nuclear and cytoplasmic positivity in most tissues throughout the body. References: The UniProt Consortium. Nucleic Acids Res. 47: D506-515 (2019); Nucleic Acids Res. 2016 Jan 4;44(D1):D733-45, PMID:26553804;
Target
Human CCNE1 / Cyclin E1
Synonyms
CCNE1 | Cyclin E1 | CCNE | Cyclin Es | Cyclin Et | G1/S-specific cyclin-E1 | G1/s-specific cyclin e | Cyclin E
Host
Rabbit
Reactivity
Human, Mouse (tested or 100% immunogen sequence identity)
Clonality
Polyclonal
Conjugations
Unconjugated
Purification
Immunoaffinity purified
Modifications
Unmodified
Immunogen
A synthetic peptide from C-Terminus of human cyclin E
Epitope
C-Terminus
Applications
  • IHC - Paraffin (10 - 15 µg/ml)
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Usage
Immunohistochemistry: LS-B3337 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B3337 was determined to be 10 ug/ml.
Presentation
10 mM PBS, pH 7.4, Sodium Azide, BSA
Storage
Store at 2°C to 8°C. Do not freeze.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee
About CCNE1 / Cyclin E1
P24864 NM_001238 NP_001229.1

Validation

CCNE1 / Cyclin E1 Antibody - Human Testis: Formalin-Fixed, Paraffin-Embedded (FFPE)
Human Testis: Formalin-Fixed, Paraffin-Embedded (FFPE)
Human Testis: Formalin-Fixed, Paraffin-Embedded (FFPE)

CCNE1 / Cyclin E1 Antibody - Human Placenta: Formalin-Fixed, Paraffin-Embedded (FFPE)
Human Placenta: Formalin-Fixed, Paraffin-Embedded (FFPE)
Human Placenta: Formalin-Fixed, Paraffin-Embedded (FFPE)

LSBio Ratings

PathPlus™ CCNE1 / Cyclin E1 Antibody (C-Terminus) for IHC LS-B3337 has an LSBio Rating of
Laboratory Validation Score (5)

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Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at:

Technical.Support@LSBio.com

Requested From: United States
Date Requested: 11/22/2024