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Catalog Number	Size	Price
LS-B8765-50	50 µg (1 mg/ml)	$605

CD3 Antibody - Anti-CD3 antibody IHC staining of human thymus. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.

PathPlus™ Monoclonal Rat anti‑Human CD3 Antibody (clone CD3‑12, IHC, WB) LS‑B8765

Name: PathPlus™ CD3 Monoclonal Antibody Rat anti-Human Flo,IHC,WB | LSBio
Brand: Lifespan BioSciences
SKU: LS-B8765-50
Price: 605.0000 USD
Availability: InStock
Rating: 5 (1 reviews)

Cancer Pathology Cancer

(5.0) Rating

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Note: This antibody replaces LS-C121496

Antibody:

CD3 Rat anti-Human Monoclonal (CD3-12) Antibody

Application:

IHC, IHC-P, IHC-Fr, WB, Flo

Reactivity:

Human

Format:

Unconjugated, Unmodified

$605

LS-B8765-50

Toll Free North America

(800) 227-6666

For Research Use Only

Specifications Validation Publications Reviews Featured Products Request SDS/MSDS

Overview

Antibody:

CD3 Rat anti-Human Monoclonal (CD3-12) Antibody

Application:

IHC, IHC-P, IHC-Fr, WB, Flo

Reactivity:

Human

Format:

Unconjugated, Unmodified

Specifications

Description

CD3 is an integral membrane glycoprotein that associates with T-cell antigen receptor (TCR) and is required for TCR cell surface expression and signal transduction. It is a highly specific marker for normal T-lymphocytes, thymocytes, and T-cell malignancies. It is negative in B-cells. References: Chetty and Gatter, 1994; Smith-Garvin et al. 2009

Target

Human CD3

See All CD3 Antibodies

Host

Rat

Reactivity

Human (tested or 100% immunogen sequence identity)

Clonality

IgG1 Monoclonal

Clone

CD3-12

Conjugations

Unconjugated

Purification

Protein G purified from tissue culture supernatant

Modifications

Unmodified

Immunogen

Synthetic peptide sequence derived from cytoplasmic epitope of CD3.

Specificity

Recognizes a highly conserved epitope of the CD3 molecule expressed by T lymphocytes.

Applications

IHC
IHC - Paraffin (10 µg/ml)
IHC - Frozen (1:1000)
Western blot (1:50 - 1:100)
Flow Cytometry (1:50 - 1:100)

Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.

Usage

Suitable for use in Flow Cytometry, Immunohistochemistry (frozen and paraffin), and Western Blot. Flow Cytometry: 1:50-1:100; 10 ul labels 10^6 cells in 100ul. Immunohistochemistry (frozen): 1:1000.

Presentation

PBS, 0.09% Sodium Azide

Storage

Short term: store at 4°C. Long term: aliquot and store at -20°C. Avoid freeze-thaw cycles.

Restrictions

For research use only. Intended for use by laboratory professionals.

Guarantee

This antibody carries the LSBio 100% Guarantee.

LSBio Guarantee

Validation

Anti-CD3 antibody IHC staining of human thymus. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.

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PathPlus™ Validation Process