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Catalog Number Size Price
LS-B5571-50 50 µl $460 
CHGA / Chromogranin A Antibody - Anti-CHGA / Chromogranin A antibody IHC of human pancreas, islets of Langerhans. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody dilution 1:100.
CHGA / Chromogranin A Antibody - Anti-CHGA / Chromogranin A antibody IHC of human small intestine, neuroendocrine cells. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody dilution 1:100.
CHGA / Chromogranin A Antibody - Anti-CHGA / Chromogranin A antibody IHC of human pancreas, islets of Langerhans. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody dilution 1:100.
CHGA / Chromogranin A Antibody - Anti-CHGA / Chromogranin A antibody IHC of human small intestine, neuroendocrine cells. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody dilution 1:100.
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PathPlus™ Monoclonal Mouse anti‑Human CHGA / Chromogranin A Antibody (clone LK2H10, Concentrated, IHC, WB) LS‑B5571

PathPlus™ Monoclonal Mouse anti‑Human CHGA / Chromogranin A Antibody (clone LK2H10, Concentrated, IHC, WB) LS‑B5571

Note: This antibody replaces LS-C134865
Antibody:
CHGA / Chromogranin A Mouse anti-Human Monoclonal (Concentrated) (LK2H10) Antibody
Application:
IHC, IHC-P, IHC-Fr, WB
Reactivity:
Human
Format:
Unconjugated, Concentrated
Price
Catalog Number
$460
LS-B5571-50
Toll Free North America
206-374-1102
For Research Use Only

Overview

Antibody:
CHGA / Chromogranin A Mouse anti-Human Monoclonal (Concentrated) (LK2H10) Antibody
Application:
IHC, IHC-P, IHC-Fr, WB
Reactivity:
Human
Format:
Unconjugated, Concentrated

Specifications

Description
CHGA (Chromogranin A) encodes a protein involved in the production of secretory vesicles in endocrine and neuroendocrine cells. Levels of this protein correlate with the number of secretory vesicles present, and staining of this protein is useful for subtyping neuroendocrine tumors in multiple tissues including the lung. Serum of patients with small cell lung cancer can be checked for circulating CHGA, and in this context it has been shown to have more sensitivity than neuroendocrine marker NSE. Sensitivity of CHGA is expected to increase with stage. Staining for Chromogranin A is expected to be cytoplasmic. References: Gut, 2016; Syversen, 2004; Taneja, 2004; Seregni, 2001; Endoc Rev 2011 32:755
Target
Human CHGA / Chromogranin A
Synonyms
CHGA | Chromogranin A | Chromogranin-A | Pancreastatin | Pituitary secretory protein I | Vasostatin I | SP-I | Vasostatin II
Host
Mouse
Reactivity
Human (tested or 100% immunogen sequence identity)
Clonality
IgG1 Monoclonal
Clone
LK2H10
Conjugations
Unconjugated
Purification
Ascites
Modifications
Concentrated
Specificity
The antibody is specific for chromogranin A. Specificity was ascertained by immunoblotting and immunohistochemistry. The antibody predominantly reacts with a 68 kD protein. Antigen was isolated from human Pheochromocytoma. Antigen location, cytoplasm. Cross-reactivity with monkey and pig.
Applications
  • IHC
  • IHC - Paraffin (1:100)
  • IHC - Frozen
  • Western blot
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Usage
This monoclonal antibody is a general marker for neurohormonal peptide producing cells (APUD) and neoplasms derived from these cells. Utrastructural studies have shown that the antigen is located in the secretory granules and therefore poorly granulated endocrine cells stain weakly or not at all. This should be considered when using this antibody. Recommended for positive control: Adrenal medulla, pancreas. The antibody can be used on acetone fixed frozen sections and formalin fixed paraffin embedded tissue. Pretreatment is not necessary. Optimal dilution should be tested by serial dilutions, 1:10 is advised based on the Indirect method. With a normal ABC method the dilution can be 5-10 times higher. Dilute immediately before use with PBS or Tris.
Presentation
Tris-HCl, 0.09% Sodium Azide
Storage
Store at 2-8°C.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee
About CHGA / Chromogranin A
P10645 NM_001275 NP_001266.1

Validation

CHGA / Chromogranin A Antibody - Anti-CHGA / Chromogranin A antibody IHC of human pancreas, islets of Langerhans. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody dilution 1:100.
Anti-CHGA / Chromogranin A antibody IHC of human pancreas, islets of Langerhans. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody dilution 1:100.
Anti-CHGA / Chromogranin A antibody IHC of human pancreas, islets of Langerhans. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody dilution 1:100.

CHGA / Chromogranin A Antibody - Anti-CHGA / Chromogranin A antibody IHC of human small intestine, neuroendocrine cells. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody dilution 1:100.
Anti-CHGA / Chromogranin A antibody IHC of human small intestine, neuroendocrine cells. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody dilution 1:100.
Anti-CHGA / Chromogranin A antibody IHC of human small intestine, neuroendocrine cells. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody dilution 1:100.

LSBio Ratings

PathPlus™ CHGA / Chromogranin A Antibody (clone LK2H10, Concentrated) for IHC, WB/Western LS-B5571 has an LSBio Rating of
Laboratory Validation Score (5)

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Requested From: United States
Date Requested: 10/9/2024