Human CHGA / Chromogranin A

Mouse

Human (tested or 100% immunogen sequence identity)

IgG1 Monoclonal

LK2H10

Unconjugated

Ascites

Concentrated

The antibody is specific for chromogranin A. Specificity was ascertained by immunoblotting and immunohistochemistry. The antibody predominantly reacts with a 68 kD protein. Antigen was isolated from human Pheochromocytoma. Antigen location, cytoplasm. Cross-reactivity with monkey and pig.

• IHC
• IHC - Paraffin (1:100)
• IHC - Frozen
• Western blot

This monoclonal antibody is a general marker for neurohormonal peptide producing cells (APUD) and neoplasms derived from these cells. Utrastructural studies have shown that the antigen is located in the secretory granules and therefore poorly granulated endocrine cells stain weakly or not at all. This should be considered when using this antibody. Recommended for positive control: Adrenal medulla, pancreas. The antibody can be used on acetone fixed frozen sections and formalin fixed paraffin embedded tissue. Pretreatment is not necessary. Optimal dilution should be tested by serial dilutions, 1:10 is advised based on the Indirect method. With a normal ABC method the dilution can be 5-10 times higher. Dilute immediately before use with PBS or Tris.

Tris-HCl, 0.09% Sodium Azide

Store at 2-8°C.

For research use only. Intended for use by laboratory professionals.

This antibody carries the LSBio 100% Guarantee.

Uniprot: P10645 NCBI: NM_001275 NP_001266.1