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| Catalog Number | Size | Price |
|---|---|---|
| LS-B3124-50 | 50 µg (1.1 mg/ml) | $495 |
1 of 2
2 of 2
PathPlus™ Monoclonal Mouse anti‑Human F8 / FVIII / Factor VIII Antibody (clone F8 2.2.9, IHC, WB) LS‑B3124
PathPlus™ Monoclonal Mouse anti‑Human F8 / FVIII / Factor VIII Antibody (clone F8 2.2.9, IHC, WB) LS‑B3124
Note: This antibody replaces LS-C82955
Antibody:
F8 / FVIII / Factor VIII Mouse anti-Human Monoclonal (F8 2.2.9) Antibody
Application:
IHC, IHC-P, WB, ELISA
Reactivity:
Human
Format:
Unconjugated, Unmodified
Toll Free North America
(800) 227-6666
(800) 227-6666For Research Use Only
Overview
Antibody:
F8 / FVIII / Factor VIII Mouse anti-Human Monoclonal (F8 2.2.9) Antibody
Application:
IHC, IHC-P, WB, ELISA
Reactivity:
Human
Format:
Unconjugated, Unmodified
Specifications
Description
Factor VIII (F8) is a coagulation factor and member of the intrinsic pathway of the coagulation cascade. It is a cofactor for Factor IXa, which converts factor X to the activated form Xa. Defects in this gene result in hemophilia A, a recessive X-linked coagulation disorder, and high expression of Factor VIII is associated with greater risk for pulmonary embolism and deep vein thrombosis. In immunohistochemistry, it is expressed in the cytoplasm of endothelial cells and in extracellular deposits in tissues throughout the body.
References: British Journal of Haematology. 2012. 157 (6): 653–63, PMID: 22530883; Invest Clin. 2016 Dec;57(4):377-87
Target
Human F8 / FVIII / Factor VIII
Synonyms
F8 | AHF | Antihemophilic factor | Coagulation factor VIIIc | Coagulation factor VIII | DXS1253E | Factor VIII F8B | FVIII | HEMA | F8B | Factor VIII | Procoagulant component | F8C
Host
Mouse
Reactivity
Human
(tested or 100% immunogen sequence identity)
Clonality
IgG1
Monoclonal
Clone
F8 2.2.9
Conjugations
Unconjugated
Purification
Protein G purified
Modifications
Unmodified
Immunogen
Affinity purified human Factor VIII.
Applications
- IHC
- IHC - Paraffin (10 µg/ml)
- Western blot
- ELISA
|
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents
ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
|
Usage
Immunohistochemistry: LS-B3124 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B3124 was determined to be 10 ug/ml.
Presentation
PBS, pH 7.4
Storage
Store at -20°C to -70°C for up to 1 year. Aliquot to avoid freeze-thaw cycles.
Restrictions
For research use only. Intended for use by laboratory professionals.
About F8 / FVIII / Factor VIII
Validation
Anti-F8 / Factor VIII antibody IHC of human uterus, vessels. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 10 ug/ml.
Anti-F8 / Factor VIII antibody IHC of human uterus, vessels. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 10 ug/ml.
Anti-F8 / Factor VIII antibody IHC of human placenta, vessels. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 10 ug/ml.
Anti-F8 / Factor VIII antibody IHC of human placenta, vessels. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 10 ug/ml.
See More About...
LSBio Ratings
PathPlus™ F8 / FVIII / Factor VIII Antibody (clone F8 2.2.9) for IHC, WB/Western, ELISA LS-B3124 has an LSBio Rating of
Laboratory Validation Score (5)
Learn more about The LSBio Ratings Algorithm
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Featured Products
Species:
Human, Mouse
Applications:
Western blot
Species:
Human
Applications:
IHC, IHC - Paraffin, ELISA
Species:
Human
Applications:
Western blot
Species:
Human
Applications:
ELISA, Immunoelectrophoresis
Species:
Pig
Applications:
Western blot
Reactivity:
Human
Range:
0.78-50 ng/ml
Request SDS/MSDS
To request an SDS/MSDS form for this product, please contact our Technical Support department at:
Technical.Support@LSBio.com
Requested From: United States
Date Requested: 11/25/2024
Date Requested: 11/25/2024
