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| Catalog Number | Size | Price |
|---|---|---|
| LS-B2157-50 | 50 µg (0.5 mg/ml) | $485 |
PathPlus™ Monoclonal Mouse anti‑Rat GAP43 Antibody (IHC, WB) LS‑B2157
PathPlus™ Monoclonal Mouse anti‑Rat GAP43 Antibody (IHC, WB) LS‑B2157
Note: This antibody replaces LS-C7321
Toll Free North America
(800) 227-6666
(800) 227-6666For Research Use Only
Overview
Specifications
Description
GAP43 (growth associated protein 43, neuromodulin, B-50) is a component of the motile growth cones at the tips of elongating axons, and it is functionally associated with nerve growth, regeneration and plasticity. It is involved in axonal and dendritic filopodia induction. It is also important for learning, memory and information storage, and heterozygous loss of the GAP43 gene results in intellectual disability. In immunohistochemistry, it has membranous and cytoplasmic positivity in the central nervous system and in peripheral nerves.
References: PLoS ONE. 9 (3): e92624, PMC: 3960265; Clinical Genetics. 53 (3): 220–2, PMID: 9630079; Front Cell Neurosci. 2017; 11: 266, PMID: 28912688
Target
Rat GAP43
Synonyms
GAP43 | Axonal membrane protein GAP-43 | B-50 | Growth associated protein 43 | Growth-associated protein 43 | Neural phosphoprotein B-50 | Neuromodulin | PP46 | GAP-43 | Protein F1
Host
Mouse
Reactivity
Rat, Human, Mouse, Rabbit, Chicken, Snake
(tested or 100% immunogen sequence identity)
Clonality
IgG2a,k
Monoclonal
Purification
Protein A purified
Modifications
Unmodified
Immunogen
Purified, full length GAP-43 protein (both phosphorylated & unphosphorylated forms).
Specificity
Recognizes rat GAP-43. Reacts with both phosphorylated and unphosphorylated forms of the GAP-43 protein.
Applications
- IHC
- IHC - Paraffin (5 µg/ml)
- IHC - Frozen (5 µg/ml)
- Western blot (1 µg/ml)
- ELISA (0.1 - 1 µg/ml)
|
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents
ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
|
Usage
Immunohistochemistry: LS-B2157 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B2157 was determined to be 5 ug/ml. Positive control: Cell lysates derived from mouse and rat brain.
Presentation
PBS, pH 7.4, 0.09% Sodium Azide
Storage
May be stored at 4°C for short-term only. Aliquot to avoid freeze-thaw cycles. Store at -20°C. Aliquots are stable for up to 1 year.
Restrictions
For research use only. Intended for use by laboratory professionals.
About GAP43
Validation
Anti-GAP43 antibody IHC of human brain, putamen, neuropil. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.
Anti-GAP43 antibody IHC of human brain, putamen, neuropil. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.
See More About...
LSBio Ratings
PathPlus™ GAP43 Antibody for IHC, WB/Western, ELISA LS-B2157 has an LSBio Rating of
Laboratory Validation Score (5)
Learn more about The LSBio Ratings Algorithm
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Featured Products
Species:
Human, Mouse, Rat
Applications:
IHC - Paraffin, Western blot
Species:
Human, Monkey, Mouse, Rat, Bovine, Chicken, Zebrafish
Applications:
IHC, IHC - Paraffin, ICC, Immunofluorescence, Western blot
Species:
Rat, Mouse
Applications:
ICC, Immunofluorescence, Western blot
Request SDS/MSDS
To request an SDS/MSDS form for this product, please contact our Technical Support department at:
Technical.Support@LSBio.com
Requested From: United States
Date Requested: 11/22/2024
Date Requested: 11/22/2024
